Cyro-hydrogel for the construction of a tyrosinase-based biosensor

被引：38

作者：

Deng, Q ^{[1
]}

Guo, YZ ^{[1
]}

Dong, SJ ^{[1
]}

机构：

[1] CHINESE ACAD SCI,CHANGCHUN INST APPL CHEM,ELECTROANALYT CHEM LAB,CHANGCHUN 130022,PEOPLES R CHINA

来源：

ANALYTICA CHIMICA ACTA | 1996年 / 319卷 / 1-2期

基金：

中国国家自然科学基金;

关键词：

biosensors; cyro-hydrogel; tyrosinase;

D O I：

10.1016/0003-2670(95)00498-X

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new immobilization method for construction of a tyrosinase based biosensor is described. A simple physical freezing technique was adopted for preparation. The immobilized enzyme yields specific activities that are more than 22% of the soluble enzyme. The enzyme electrode can be stored in dry state for more than three months without any loss of activity. The biosensor was applied to the determination of several phenols and o-diphenols. The lowest detect limit is 0.02 mu mol/1 and the linear range was 1.0 X 10(-7)-1.0 X 10(-4) mol/1 for catechol. The kinetic parameters have also been calculated.

引用

页码：71 / 77

页数：7

共 17 条

[1] BIOAMPEROMETRIC SENSORS FOR PHENOL BASED ON CARBON PASTE ELECTRODES [J].

BONAKDAR, M ;

VILCHEZ, JL ;

MOTTOLA, HA .

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1989, 266 (01) :47-55

[2] A NEW STRATEGY FOR THE CONSTRUCTION OF A TYROSINASE-BASED AMPEROMETRIC PHENOL AND O-DIPHENOL SENSOR [J].

COSNIER, S ;

INNOCENT, C .

BIOELECTROCHEMISTRY AND BIOENERGETICS, 1993, 31 (02) :147-160

[3]

DONG SJ, 1994, J ELECTROANAL CHEM, V375, P405

[4]

FENG YD, 1989, J SICHUAN U, V26, P470

[5] FREEZING AND NONFREEZING WATER IN CELLULOSE-ACETATE MEMBRANES [J].

FROMMER, MA ;

LANCET, D .

JOURNAL OF APPLIED POLYMER SCIENCE, 1972, 16 (05) :1295-&

[6]

GIBSON TD, 1992, BIOSENSORS CHEM SENS, pCH5

[7]

HUANG G, 1992, ACTA POLYM SINICA, V3, P6

[8] ROTATING-RING-DISK ENZYME ELECTRODE FOR BIOCATALYSIS KINETIC-STUDIES AND CHARACTERIZATION OF THE IMMOBILIZED ENZYME LAYER [J].

KAMIN, RA ;

WILSON, GS .

ANALYTICAL CHEMISTRY, 1980, 52 (08) :1198-1205

[9] MUSHROOM POLYPHENOL OXIDASE .I. PURIFICATION AND GENERAL PROPERTIES [J].

KERTESZ, D ;

ZITO, R .

BIOCHIMICA ET BIOPHYSICA ACTA, 1965, 96 (03) :447-&

[10] PLANT-METABOLISM AS AN ANALYTICAL TOOL - SOME APPLICATIONS OF PLANT-TISSUE ELECTRODES FOR THE SELECTIVE DETERMINATION OF CATECHOLAMINES [J].

MAZZEI, F ;

BOTRE, F ;

LANZI, M ;

LORENTI, G ;

PORCELLI, F ;

BOTRE, C .

SENSORS AND ACTUATORS B-CHEMICAL, 1992, 7 (1-3) :427-430

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