Overexpression of the Anthocyanidin Synthase Gene in Strawberry Enhances Antioxidant Capacity and Cytotoxic Effects on Human Hepatic Cancer Cells

被引：87

作者：

Giampieri, Francesca ^{[1
]}

Gasparrini, Massimiliano ^{[1
]}

Forbes-Hernandez, Tamara Y. ^{[1
,3
]}

Mazzoni, Luca ^{[2
]}

Capocasa, Franco ^{[2
]}

Sabbadini, Silvia ^{[2
]}

Alvarez-Suarez, Jose M. ^{[4
]}

Afrin, Sadia ^{[1
]}

Rosati, Carlo ^{[5
]}

Pandolfini, Tiziana ^{[6
]}

Molesini, Barbara ^{[6
]}

Sanchez-Sevilla, Jose F. ^{[7
]}

Amaya, Iraida ^{[7
]}

Mezzetti, Bruno ^{[2
]}

Battino, Maurizio ^{[1
,8
]}

机构：

[1] Univ Politecn Marche, Fac Med, Sez Biochim, Dipartimento Sci Clin Specialist & Odontostomatol, Via Ranieri 65, I-60131 Ancona, Italy

[2] Univ Politecn Marche, Dipartimento Sci Agr, Via Ranieri 65, I-60131 Ancona, Italy

[3] Univ Int Iberoamer UNINI, Area Nutr & Salud, Campeche 24040, Mexico

[4] Univ Las Amer, Fac Ciencias Salud, Grp Invest Biotecnol Aplicada Biomed BIOMED, Campus Queri Bloque 7 Planta Baja,EC170125, Quito, Ecuador

[5] ENEA Trisaia Res Ctr, SS 106 Km 419-5, I-75026 Rotondella, Matera, Italy

[6] Univ Verona, Dipartimento Biotecnol, I-37129 Verona, Italy

[7] IFAPA Ctr Churriana, Inst Andaluz Invest & Format Agr & Pesquera, Cortijo de la Cruz S-N, Malaga 29140, Spain

[8] UEA, Ctr Nutr & Hlth, Santander 39011, Spain

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2018年 / 66卷 / 03期

关键词：

anthocyanidin synthase; anticancer effects; apoptosis; flavonoids; RNA-seq; strawberry; transcriptome; ROS; mitochondrial functionality; HEALTH-BENEFITS; IN-VITRO; BIOSYNTHESIS; INHIBITION; EXPRESSION; APOPTOSIS; GLUCOSYLTRANSFERASE; IDENTIFICATION; MECHANISMS; GROWTH;

D O I：

10.1021/acs.jafc.7b04177

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Food fortification through the increase and/or modulation of bioactive compounds has become a major goal for preventing several diseases, including cancer. Here, strawberry lines of cv. Calypso transformed with a construct containing an anthocyanidin synthase (ANS) gene were produced to study the effects on anthotyanin biosynthesis, metabolism, and transcriptome. Three strawberry ANS transgenic lines (ANS L5, ANS L15, and ANS L18) were analyzed for phytochemical composition and total antioxidant capacity (TAC), and their fruit extracts were assessed for cytotoxic effects on hepatocellular carcinoma. ANS L18 fruits had the highest levels of total phenolics and flavonoids, while those of ANS L15 had the highest anthocyanin concentration; TAC positively correlated with total polyphenol content. Fruit transcriptome was also specifically affected in the polyphenol biosynthesis and in other related metabolic pathways. Fruit extracts of all lines exerted cytotoxic effects in a dose/time-dependent manner, increasing cellular apoptosis and free radical levels and impairing mitochondrial functionality.

引用

页码：581 / 592

页数：12

共 47 条

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