Rapid and sensitive detection of Lily symptomless virus by reverse transcription loop-mediated isothermal amplification

被引：14

作者：

He, Xiangfeng ^{[1
,2
]}

Xue, Fei ^{[3
]}

Xu, Shufa ^{[3
]}

Wang, Wenhe ^{[1
,2
]}

机构：

[1] Beijing Agr Univ, Coll Landscape Architecture, Beijing Engn Res Ctr Rural Landscape Planning & D, Beijing 102206, Peoples R China

[2] Beijing Collaborat Innovat Ctr Ecoenvironm Improv, Beijing 102206, Peoples R China

[3] Chinese Acad Agr Sci, Inst Apicultural Res, Minist Agr, Key Lab Pollinating Insect Biol, Beijing 100093, Peoples R China

来源：

JOURNAL OF VIROLOGICAL METHODS | 2016年 / 238卷

关键词：

Lilium spp; RT-LAMP; LSV; Detection; ASSAY;

D O I：

10.1016/j.jviromet.2016.10.003

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Lily symptomless virus (LSV) is one of the most prevalent viruses that infect lily plants worldwide. A rapid and sensitive reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for detection of LSV, using two primer pairs that specifically amplified the conserved sequence of LSV coat protein. The optimum reaction conditions were as follows: 4 mM MgCl2 and 0.8 M betaine with incubation at 64 degrees C for 30 min. The limit of detection of LSV from infected lily leaves was 10-fold higher for RT-LAMP than for conventional RT-PCR. Moreover, RT-LAMP detected LSV in not only symptomatic, but also in symptomless tissues of infected plants. These findings indicate that our RT-LAMP method for LSV can serve as a low-cost, simple, and rapid alternative to conventional detection assays. (C) 2016 Elsevier B.V. All rights reserved.

引用

页码：38 / 41

页数：4

共 15 条

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