Characterization of the effects of defined, multidimensional culture conditions on conditionally reprogrammed primary human prostate cells

被引：17

作者：

Tricoli, Lucas ^{[1
,4
]}

Naeem, Aisha ^{[1
]}

Parasido, Erika ^{[1
]}

Mikhaiel, John P. ^{[1
]}

Choudhry, Muhammad Umer ^{[1
]}

Berry, Deborah L. ^{[1
]}

Abdelgawad, Iman A. ^{[2
]}

Lee, Richard J. ^{[3
]}

Feldman, Adam S. ^{[3
]}

Ihemelandu, Chukwuemeka ^{[1
]}

Avantaggiati, Maria ^{[1
]}

Kumar, Deepak ^{[4
]}

Byers, Stephen ^{[1
]}

Gallagher, Rosa ^{[5
]}

Wulfkuhle, Julia ^{[5
]}

Petricoin, Emanuel ^{[5
]}

Rodriguez, Olga ^{[1
,6
]}

Albanese, Chris ^{[1
,6
]}

机构：

[1] Georgetown Univ, Med Ctr, Dept Oncol, Lombardi Comprehens Canc Ctr, Washington, DC 20007 USA

[2] Natl Canc Inst Egypt, Cairo, Egypt

[3] Massachusetts Gen Hosp, Ctr Canc, Boston, MA USA

[4] North Carolina Cent Univ, Julius L Chambers Biomed Biotechnol Res Inst, Durham, NC USA

[5] George Mason Univ, Ctr Appl Prote & Mol Med, Manassas, VA USA

[6] Georgetown Univ, Med Ctr, Preclin Imaging Res Lab, Washington, DC 20007 USA

来源：

ONCOTARGET | 2018年 / 9卷 / 02期

关键词：

prostate; cancer; primary tissue; reprogrammed cells; androgen; ANDROGEN RECEPTOR; EPITHELIAL-CELLS; CANCER CELLS; MICROARRAY DATA; ROCK INHIBITOR; SMOOTH-MUSCLE; STRIGOLACTONE; APOPTOSIS; ANALOGS; MODELS;

D O I：

10.18632/oncotarget.23363

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The inability to propagate human prostate epithelial cells indefinitely has historically presented a serious impediment to prostate cancer research. The conditionally reprogrammed cell (CRC) approach uses the combination of irradiated J2 mouse fibroblasts and a Rho kinase inhibitor such as Y27632 to support the continuous culture of cells derived from most epithelial tissues, including the prostate. Due to their rapid establishment and overall ease of use, CRCs are now widely used in a variety of basic and preclinical settings. In addition, CRCs were successfully used to clinically treat respiratory papillomatosis. Although both normal and tumor-derived prostate CRCs have been used to study the basic biology of prostate cancer and to test new therapies, certain limitations exist. We have previously reported that prostate CRCs form functional prostate glands when implanted under the mouse renal capsule. However in conventional culture, the prostate CRCs exist in an adult stem-like, transient amplifying state and consequently do not adequately recapitulate several important features of a differentiated prostate epithelium. To address these limitations, we previously described a transwell dish-based model that supported the culturing of prostate CRCs and the collection of cells and cell extracts for molecular and genetic analyses. Using normal and tumor-derived prostate CRCs, we describe the combined effects of the multi-dimensional transwell platform and defined culture media on prostate cellular proliferation, differentiation and signaling.

引用

页码：2193 / 2207

页数：15

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