Eucalyptol reduces airway hyperresponsiveness in rats following cigarette smoke-exposed

被引:6
作者
Kennedy-Feitosa, Emanuel [1 ]
Oliveira-Melo, Paolo [2 ]
Evangelista-Costa, Eder [2 ]
Serra, Daniel Silveira [2 ]
Avila Cavalcante, Francisco Sales [2 ]
da Ponte, Edson Lopes [3 ]
Barbosa, Roseli [4 ]
Rodrigues da Silva, Renata Evaristo [4 ]
Sampaio Assreuy, Ana Maria [2 ]
Leal-Cardoso, Jose Henrique [2 ]
Lima, Crystianne Calado [2 ]
机构
[1] Univ Fed Rural Semi Arido, Dept Ciencias Saude, UFERSA, R Francisco Mota,572 Pres Costa E Silva, BR-59625900 Mossoro, RN, Brazil
[2] Univ Estadual Ceara, Inst Super Ciencias Biomed, UECE, Campina Grande, Paraiba, Brazil
[3] Unichristus, Ctr Univ Christus, Fortaleza, Ceara, Brazil
[4] Univ Reg Cariri, Dept Quim Biol, URCA, Crato, CE, Brazil
关键词
Eucalyptol; Hyperresponsiveness; Lung function; Inflammation; OXIDATIVE STRESS; SMOOTH-MUSCLE; INFLAMMATION; LUNG;
D O I
10.1016/j.pupt.2020.101887
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Background: Cigarette smoke is the major cause of airway inflammatory disease, including airway hyperresponsiveness. Eucalyptol (EUC), also named 1.8-cineole, is a monoterpenoid found in essential oil of medicinal plants, showing several biological effects. Hypothesis/purpose: Based in the eucalyptol protective activity in respiratory diseases as asthma, our hypothesis is that eucalyptol is able to reduce the airway hyperresponsiveness and the respiratory mechanic parameters in rats exposed to cigarette smoke. Study design: Wistar rats were divided into control and cigarettes smoke (CS) groups. CS group was daily subjected to cigarette smoke and treated by inhalation for 15 min/day with EUC (1 mg/mL) or vehicle during 30 days. After treatment, bronchoalveolar lavage (BAL) was collected to analyze the inflammatory profile, and tracheal rings were isolated for evaluation of the airway smooth muscle hyperresponsiveness. Lung function was analyzed in vivo. Methods: The inflammatory profile was evaluated by optical microscopy performing total (Neubauer chamber) and differential leukocyte count (smear slides stained in H&E). The hyperresponsiveness was evaluated in tracheal rings contracted with potassium chloride (KCl) carbamoylcholine (CCh), or Barium chloride (BaCl2) in presence or absence of nifedipine. The lung function (Newtonian resistance-R-N) was evaluated by bronco stimulation with methacholine (MCh). Results: BAL from CS group increased the influx of leukocyte, mainly neutrophils and macrophages compared to control group. EUC reduced by 71% this influx. The tracheal contractions induced by KCl, CCh or BaCl2 were reduced by EUC in 59%, 42% and 26%, respectively. The last one was not different of nifedipine activity. Newtonian resistance (R-N) was also reduced in 37% by EUC compared to CS group. Conclusion: EUC reduces the hyperresponsiveness and the airway inflammatory profile, recovering the lung function.
引用
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页数:7
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