Identification and Chemical Standardization of Licorice Raw Materials and Dietary Supplements Using UHPLC-MS/MS

被引:71
作者
Li, Guannan [1 ]
Nikolic, Dejan [1 ]
van Breemen, Richard B. [1 ]
机构
[1] Univ Illinois, UIC NIH Ctr Bot Dietary Supplements Res, Chicago Mass Spectrometry Lab, Dept Med Chem & Pharmacognosy,Coll Pharm, Chicago, IL 60612 USA
基金
美国国家卫生研究院;
关键词
botanical dietary supplements; licorice; method of standard addition; tandem mass spectrometry; UHPLC; GLYCYRRHIZA-GLABRA; BIOACTIVE COMPONENTS; RADIX GLYCYRRHIZAE; MASS-SPECTROMETRY; ISOLIQUIRITIGENIN; LICOCHALCONE; SEPARATION; CHEMISTRY; URALENSIS; EUROPE;
D O I
10.1021/acs.jafc.6b02954
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
Defined as the roots and underground stems of principally three Glycyrrhiza species, Glycyrrhiza glabra L., Glycyrrhiza uralensis Fish. ex DC., and Glycyrrhiza inflata Batalin, licorice has been used as a medicinal herb for millennia and is marketed as root sticks, powders, and extracts. Identity tests described in most pharmacopeial monographs enabled the distinction of Glycyrrhiza species. Accordingly, an ultrahigh-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) assay using the method of standard addition was developed to quantify 14 licorice components (liquiritin, isoliquiritin, liquiritin apioside, isoliquiritin apioside, licuraside, liquiritigenin, isoliquiritigenin, glycyrrhizin, glycyrrhetinic acid, glabridin, glycycoumarin, licoricidin, licochalcone A, and beta-hydroxybenzylmalonic acid), representing several natural product classes including chalcones, flavanones, saponins, and isoflavonoids. Using this approach, G. glabra, G. uralensis, and G. inflata in a variety of forms including root powders and extracts as well as complex dietary supplements could be differentiated and chemically standardized without concerns due to matrix effects.
引用
收藏
页码:8062 / 8070
页数:9
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