Molecular Basis Underlying the Biological Effects Elicited by Extremely Low-Frequency Magnetic Field (ELF-MF) on Neuroblastoma Cells

被引:46
作者
Sulpizio, Marilisa [1 ,2 ]
Falone, Stefano [1 ]
Amicarelli, Fernanda [3 ]
Marchisio, Marco [2 ,4 ]
Di Giuseppe, Fabrizio [1 ,2 ]
Eleuterio, Enrica [1 ,2 ]
Di Ilio, Carmine [1 ,2 ]
Angelucci, Stefania [1 ,2 ]
机构
[1] Univ G dAnnunzio, Dept Biomed Sci, I-66013 Chieti, Italy
[2] Univ G dAnnunzio, Ctr Excellence Aging, Chieti, Italy
[3] Univ Aquila, Dept Basic & Appl Biol, Laquila, Italy
[4] Univ G dAnnunzio, Dept Med & Aging Sci, I-66013 Chieti, Italy
关键词
50-HZ MAGNETIC FIELDS; NEUROBLASTOMA; PROTEOME; HUMAN HEALTH; PROLIFERATION; DIFFERENTIATION; ELECTROMAGNETIC-FIELDS; IN-VITRO; PROTEOMIC ANALYSIS; HYDROGEN-PEROXIDE; INDUCED APOPTOSIS; PEROXIREDOXIN-VI; SULFANE SULFUR; RETINOIC ACID; DNA-DAMAGE; EXPOSURE;
D O I
10.1002/jcb.23310
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Extremely low-frequency magnetic fields (ELF-MFs) may affect human health because of the possible associations with leukemia but also with cancer, cardiovascular, and neurological disorders. In the present work, human SH-SY5Y neuroblastoma cells were exposed to a 50 Hz, 1mT sinusoidal ELF-MF at three different times, that is, 5 days (T5), 10 days (T10), and 15 days (T15) and then the effects of ELF-MF on proteome expression and biological behavior were investigated. Through comparative analysis between treated and control samples, we analyzed the proteome changes induced by ELF-MF exposure. Nine new proteins resolved in sample after a 15-day treatment were involved in a cellular defense mechanism and/or in cellular organization and proliferation such as peroxiredoxin isoenzymes (2, 3, and 6), 3-mercaptopyruvate sulfurtransferase, actin cytoplasmatic 2, t-complex protein subunit beta, ropporin-1A, and profilin-2 and spindlin-1. Our results indicated that ELF-MFs exposure altered the proliferative status and other important cell biology-related parameters, such as cell growth pattern, and cytoskeletal organization. These findings support our hypothesis that ELF radiation could trigger a shift toward a more invasive phenotype. J. Cell. Biochem. 112: 3797-3806, 2011. (C) 2011 Wiley Periodicals, Inc.
引用
收藏
页码:3797 / 3806
页数:10
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