Genetic diversity of the common bean Phaseolus vulgaris L determined by DNA-based molecular markers

被引:0
作者
Vasconcelos, MJV [1 ]
deBarros, EG [1 ]
Moreira, MA [1 ]
Vieira, C [1 ]
机构
[1] EMBRAPA,CTR NACL PESQUISA MILHO & SORGO,BR-35701970 SETE LAGOAS,MG,BRAZIL
来源
BRAZILIAN JOURNAL OF GENETICS | 1996年 / 19卷 / 03期
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中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
We selected 28 potential common bean (Phaseolus vulgaris L.) progenitors to determine their genetic diversity using RAPD technique. They were divided into two groups based on their type of storage protein (phaseolin); 18 of them had type ''S'' phaseolin and the other 10 had type ''T'' phaseolin. DNA samples of all the cultivars were amplified in vitro with oligonucleotide primers of random sequences. A total of 276 amplification products, 144 of them being polymorphic, were obtained with 45 different primers. Multivariate analysis of these data divided the cultivars into two groups and, in addition, allowed a more precise intra and intergroup distinction.
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页码:447 / 451
页数:5
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