Effects of an Escherichia coli exopolysaccharide on human and mouse gut microbiota in vitro

被引:9
作者
Li, Baiyuan [1 ]
Chen, Huahai [1 ]
Cao, Linyan [1 ]
Hu, Yunfei [1 ]
Chen, Dan [1 ]
Yin, Yeshi [1 ,2 ]
机构
[1] Hunan Univ Sci & Engn, Coll Chem & Bioengn, Key Lab Comprehens Utilizat Advantage Plants Reso, Yongzhou, Hunan, Peoples R China
[2] Zhejiang Acad Agr Sci, Inst Plant Protect & Microbiol, State Key Lab Breeding Base Zhejiang Sustainable, Hangzhou, Zhejiang, Peoples R China
基金
湖南省自然科学基金;
关键词
EPS-RB; Gut microbiota; High-throughput sequencing; Short-chain fatty acid; POLYSACCHARIDE; FERMENTATION; COMMUNITY; STRAINS; GROWTH; TRACT;
D O I
10.1016/j.ijbiomac.2019.10.186
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In this study, an exopolysaccharide (EPS) named EPS-RB was produced when the gene cluster ycjD-fabl-yciW-rnb were overexpressed in E. coli. Monosaccharide composition analysis revealed that EPS-RB is a novel EPS that consisted of L-fucose, L-arabinose, D-galactose/N-acetyl glucosamine, D-glucose, D-xylose, D-ribose, and D-glucuronic add, and their molecular ratio was approximately 80:3:53:69:1:2:64. The content of carbohydrates, protein, and uronic adds in EPS-RB was 90.35 +/- 135%, 2.62 +/- 0.05% and 8.16 +/- 1.00%, respectively. The interaction between EPS-RB and gut microbiota was investigated using an in vitro batch fermentation system. The results showed that -96% of EPS-RB can be degraded by human fecal microbiota after 72 h fermentation, but few can be degraded by mouse cecal microbiota. Furthermore, high-throughput sequencing showed that EPS-RB regulates the human gut microbiota. The genera Collinsella, Butyricimonas, and Hafnia were enriched in group VIR (EPS-RB as a carbon source) when compared with group VI (no carbon source) and VIS (starch as a carbon source). Short-chain fatty acids (SCFAs) production analysis showed that their concentration was significantly higher in group VIR than groups VI and VIS after 72 h fermentation. In summary, an EPS-RB in E. coli was isolated and its regulatory function on gut microbiota was analyzed. (C) 2019 Elsevier B.V. All rights reserved.
引用
收藏
页码:991 / 999
页数:9
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