Genetic variation within and among five natural populations of endangered Sclerocarya birrea (A. Rich) subsp Birrea in Sudan

被引：0

作者：

Abdelkheir, Rehab M. ^{[2
]}

Hamza, Nada B. ^{[1
]}

Khalil, Ayoub M. ^{[2
]}

Warrag, Essam I. ^{[3
]}

机构：

[1] Natl Res Ctr, Dept Mol Biol, Commiss Biotechnol & Genet Engn, Khartoum, Sudan

[2] Univ Juba, Dept Forestry, Coll Nat Resources & Environm Studies, Khartoum, Sudan

[3] Univ Khartoum, Fac Forestry, Khartoum 11115, Sudan

来源：

AFRICAN JOURNAL OF BIOTECHNOLOGY | 2011年 / 10卷 / 28期

关键词：

Sclerocarya birrea subsp birrea; genetic variation; conservation; random amplified polymorphic DNA (RAPD); Sudan; RAPD VARIATION; DIVERSITY; DNA; TREE; DIFFERENTIATION; CONSERVATION; PATTERNS; NUCLEAR; AFRICA;

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Knowledge of genetic diversity is important for successful conservation and domestication of species. In order to determine genetic diversity within and among Sclerocarya birrea (A. Rich.) Htochst. subsp. birrea populations in Sudan, random amplified polymorphic DNA (RAPD) markers were used. Leaf materials from 75 seedlings from five populations (Rashad, Alfaid, Alkhwi, Aldamazin and Baw) of this species were used to compare the genetic diversity. A total of 37 bands were generated using four primers. Genetic variation within the populations as estimated by Shannon information index ranged from 0.343 to 0.272 with an overall diversity of 0.306. Analysis of molecular variance revealed that 46% of the variation was attributed to differences among the populations and 54% within the populations (P < 0.001). The gene flow among population was small (Nm = 0.297). UPGMA cluster and principal coordinate analyses (PCA) indicated Alfaid as the most distinct population. Since genetic variation was found to occur among and within the populations of S. birrea (A. Rich.) Hochst. Subsp. birrea, conservation of these populations would help in the maintenance of the species.

引用

页码：5452 / 5460

页数：9

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