Fibronectin is a binding partner for the myelin-associated glycoprotein (siglec-4a)

被引：21
作者：
Strenge, K
Brossmer, R
Ihrig, P
Schauer, R
Kelm, S
机构：
[1] Univ Bremen, Dept Biol & Chem, D-28334 Bremen, Germany
[2] Univ Kiel, Inst Biochem, D-24098 Kiel, Germany
[3] Heidelberg Univ, Biochem Ctr Heidelberg BZH, D-69120 Heidelberg, Germany
来源：
FEBS LETTERS | 2001年 / 499卷 / 03期
关键词：
myelin-associated glycoprotein; siglec; sialic acid; cellular interaction; extracellular matrix; fibronectin;
D O I：
10.1016/S0014-5793(01)02566-2
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The myelin-associated glycoprotein (MAG) mediates cell-cell interactions between myelinating glial cells and neurons. Here we describe the extracellular matrix glycoprotein fibronectin as a binding partner of MAG. It has been identified by affinity precipitation with MAG-Fe from NG108-15 cells and by microsequencing of two peptides derived from a 210-kDa protein band. Western blot analysis showed that fibronectin is also present in;MAG binding partners isolated from N(2)A (murine neuroblastoma) cells, rat brain and rat spinal cord, Different fibronectin isoforms have been isolated from brains of young and adult rats, indicating that the expression of MAG binding fibronectin changes during development. (C) 2001 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.
引用
收藏
页码：262 / 267
页数：6
相关论文
共 44 条
[1]   RECOGNITION MOLECULES MYELIN-ASSOCIATED GLYCOPROTEIN AND TENASCIN-C INHIBIT INTEGRIN-MEDIATED ADHESION OF NEURAL CELLS TO COLLAGEN [J].
BACHMANN, M ;
CONSCIENCE, JF ;
PROBSTMEIER, R ;
CARBONETTO, S ;
SCHACHNER, M .
JOURNAL OF NEUROSCIENCE RESEARCH, 1995, 40 (04) :458-470
[2]  
BROSSMER R, 1994, METHOD ENZYMOL, V247, P153
[3]  
Crocker P R, 1998, Glycobiology, V8, pv
[4]   Sialoadhesin and related cellular recognition molecules of the immunoglobulin superfamily [J].
Crocker, PR ;
Kelm, S ;
Hartnell, A ;
Freeman, S ;
Nath, D ;
Vinson, M ;
Mucklow, S .
BIOCHEMICAL SOCIETY TRANSACTIONS, 1996, 24 (01) :150-156
[5]  
De Bellard M, 1999, J NEUROSCI RES, V56, P213, DOI 10.1002/(SICI)1097-4547(19990415)56:2<213::AID-JNR11>3.0.CO
[6]  
2-U
[7]   Myelin-associated glycoprotein inhibits axonal regeneration from a variety of neurons via interaction with a sialoglycoprotein [J].
DeBellard, ME ;
Tang, S ;
Mukhopadhyay, G ;
Shen, YJ ;
Filbin, MT .
MOLECULAR AND CELLULAR NEUROSCIENCE, 1996, 7 (02) :89-101
[8]   CHARACTERIZATION OF BINDING-PROPERTIES OF THE MYELIN-ASSOCIATED GLYCOPROTEIN TO EXTRACELLULAR-MATRIX CONSTITUENTS [J].
FAHRIG, T ;
LANDA, C ;
PESHEVA, P ;
KUHN, K ;
SCHACHNER, M .
EMBO JOURNAL, 1987, 6 (10) :2875-2883
[9]   ALTERNATIVE SPLICING OF FIBRONECTIN - MANY DIFFERENT PROTEINS BUT FEW DIFFERENT FUNCTIONS [J].
FFRENCHCONSTANT, C .
EXPERIMENTAL CELL RESEARCH, 1995, 221 (02) :261-271
[10]   CRUCIAL ROLE FOR THE MYELIN-ASSOCIATED GLYCOPROTEIN IN THE MAINTENANCE OF AXON-MYELIN INTEGRITY [J].
FRUTTIGER, M ;
MONTAG, D ;
SCHACHNER, M ;
MARTINI, R .
EUROPEAN JOURNAL OF NEUROSCIENCE, 1995, 7 (03) :511-515
12345