Comparisons between continuous and batch processing to produce clavulanic acid by Streptomyces clavuligerus

被引：9

作者：

Baptista-Neto, I ^{[1
]}

Teodoro, JC ^{[1
]}

Cassiano, LCM ^{[1
]}

Badino, AC ^{[1
]}

Hokka, CO ^{[1
]}

机构：

[1] Univ Fed Sao Carlos, Dept Engn Quim, BR-13565905 Sao Carlos, SP, Brazil

来源：

BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY | 2005年 / 48卷

关键词：

clavulanic acid; Streptomyces clavuligerus and continuous culture;

D O I：

10.1590/S1516-89132005000400012

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The aim of the present work was to compare CA production in continuous culture with and without cell recycling and in batch process by Streptomyces clavuligerus. Continuous cultivations with high cell concentration using cell recycling were performed utilizing a hollow fiber ultrafiltration module to separate cells from the filtrate broth. The continuous cultures without cell recycling and the batch cultivations were performed conventionally. The highest productivity was attained in the continuous cultivation with cell recycling (22.2 mg.L-1.h(-1)). The highest CA concentration was obtained in the batch process (470 mg.L-1.h(-1)).

引用

页码：97 / 104

页数：8

共 9 条

[1] Chemistry and biosynthesis of clavulanic acid and other clavams [J].

Baggaley, KH ;

Brown, AG ;

Schofield, CJ .

NATURAL PRODUCT REPORTS, 1997, 14 (04) :309-333

[2]

BARSI FV, 2003, C BRAS ENG QUIM IN C, V5

[3]

BLACH HW, 1997, BIOCH ENG, P276

[4]

BUTTERWORTH D, 1984, BIOTECHNOLOGY IND AN, V22, P255

[5] ASSAY OF AMOXICILLIN AND CLAVULANIC ACID, THE COMPONENTS OF AUGMENTIN, IN BIOLOGICAL-FLUIDS WITH HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

FOULSTONE, M ;

READING, C .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1982, 22 (05) :753-762

[6] Improvement of clavulanic acid production by Streptomyces clavuligerus in medium containing soybean derivatives [J].

Gouveia, ER ;

Baptista-Neto, A ;

Azevedo, AG ;

Badino, AC ;

Hokka, CO .

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 1999, 15 (05) :623-627

[7] Growth limiting substrate affects antibiotic production and associated metabolic fluxes in Streptomyces clavuligerus [J].

Kirk, S ;

Avignone-Rossa, CA ;

Bushell, ME .

BIOTECHNOLOGY LETTERS, 2000, 22 (22) :1803-1809

[8] Cultivation of Thermoanaerobium brockii in continuous culture with complete cell recycling [J].

Manelius, A ;

Holst, O .

LETTERS IN APPLIED MICROBIOLOGY, 1997, 24 (05) :384-388

[9]

SHULER ML, 1992, BIOPROCESS ENG, P104

← 1 →