Crystallization and preliminary X-ray crystallographic analysis of extracellular giant hemoglobin from pogonophoran Oligobrachia mashikoi

被引：6
|
作者：
Numoto, N
Nakagawa, T
Kita, A
Sasayama, Y
Fukumori, Y
Miki, K [1 ]
机构：
[1] Kyoto Univ, Grad Sch Sci, Dept Chem, Sakyo Ku, Kyoto 6068502, Japan
[2] RIKEN, Harima Inst, SPring 8, Sayogun, Hyogo 6795148, Japan
[3] Kanazawa Univ, Grad Sch Nat Sci & Technol, Dept Life Sci, Kanazawa, Ishikawa 9201192, Japan
[4] Kyoto Univ, Inst Res Reactor, Kumatori, Osaka 5900494, Japan
[5] Kanazawa Univ, Inst Nat & Environm Technol, Noto Marine Lab, Div Biodivers, Uchiura, Ishikawa 9270553, Japan
来源：
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS | 2005年 / 1750卷 / 02期
关键词：
extracellular hemoglobin; invertebrate; pogonophora; Oligobrachia mashikoi; sulfide binding;
D O I：
10.1016/j.bbapap.2005.05.009
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
An extracellular giant hemoglobin of Oligobrachia mashikoi, composed of 24 globins with the molecular mass of similar to 400 kDa was crystallized in its intact form. Two crystal forms were obtained by the vapor-diffusion method. Form I crystals obtained using sodium acetate as a precipitant belong to the space group P6(1)22 or P6(5)22, with unit-cell parameters a = 112.41, c = 621.25 angstrom, and diffracted X-rays beyond 3.0 angstrom resolution. Form II crystals obtained using PEG 10000 as a precipitant belong to the space group R32, with unit-cell parameters a = 111.50, c = 276.84 angstrom, and diffracted X-rays beyond 2.9 angstrom resolution. The crystals are suitable for X-ray crystallography to determine the supramacromolecular assembly of this giant hemoglobin. (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码：173 / 176
页数：4
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