High-efficiency cell disruption and astaxanthin recovery from Haematococcus pluvialis cyst cells using room-temperature imidazoliumbased ionic liquid/water mixtures

被引：70

作者：

Choi, Sun-A ^{[1
,2
]}

Oh, You-Kwan ^{[3
]}

Lee, Jiye ^{[1
,3
]}

Sim, Sang Jun ^{[2
]}

Hong, Min Eui ^{[2
]}

Park, Ji-Yeon ^{[1
]}

Kim, Min-Sik ^{[1
]}

Kim, Seung Wook ^{[2
]}

Lee, Jin-Suk ^{[1
]}

机构：

[1] Korea Inst Energy Res, Climate Change Res Div, Daejeon 34129, South Korea

[2] Korea Univ, Dept Chem & Biol Engn, Seoul 02841, South Korea

[3] Pusan Natl Univ, Sch Chem & Biomol Engn, Busan 46241, South Korea

来源：

BIORESOURCE TECHNOLOGY | 2019年 / 274卷

关键词：

Haematococcus pluvialis cyst; Ionic liquid; Astaxanthin extraction; Lipid extraction; Cell disruption; LIPID EXTRACTION; WALL DISRUPTION; CHLORELLA; CULTIVATION; IMPROVEMENT; LIQUIDS; ACID;

D O I：

10.1016/j.biortech.2018.11.082

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

Energy-saving, high-efficiency cell disruption is a critical step for recovery of thermolabile antioxidant astaxanthin from Haematococcus pluvialis cyst cells of rigid cell-wall structure. In this study, as room-temperature green solvents, 10 types of 1-ethyl-3-methylimidazolium([Emim])-based ionic liquids (ILs) were compared and evaluated for their abilities to disrupt H. pluvialis cyst cells for astaxanthin/lipid extraction. Among the 10 ILs tested, 3 [Emim]-based ILs with HSO4, CH3SO3, and (CF3SO2)(2)N anions were selected based on astaxanthin/lipid extraction performance and synthesis cost. When pretreated with IL/water mixtures, intact cyst cells were significantly torn, broken or shown to release cytoplasmic components, thereby facilitating subsequent separation of astaxanthin/lipid by hexane. However, excess IL pretreatments at high temperature/IL dosages and longer incubation times significantly deteriorated lipid and/or astaxanthin. Under optimized mild conditions (6.7% (v/v) IL in water solution, 30 degrees C, 60 min), almost complete astaxanthin recoveries (> 99%) along with moderate lipid extractions (similar to 82%) could be obtained.

引用

页码：120 / 126

页数：7

共 34 条

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