Spectral Doppler optical coherence tomography imaging of localized ischemic stroke in a mouse model

被引：25

作者：

Yu, Lingfeng ^{[1
]}

Nguyen, Elaine ^{[1
]}

Liu, Gangjun ^{[1
]}

Choi, Bernard ^{[1
]}

Chen, Zhongping ^{[1
]}

机构：

[1] Univ Calif Irvine, Beckman Laser Inst, Dept Biomed Engn, Irvine, CA 92612 USA

来源：

JOURNAL OF BIOMEDICAL OPTICS | 2010年 / 15卷 / 06期

基金：

美国国家卫生研究院;

关键词：

Doppler; biomedical optics; laser speckle imaging; laser Doppler velocimetry; tomography; BLOOD-FLOW; INTRINSIC SIGNALS; CEREBRAL-ISCHEMIA; ANGIOGRAPHY; VELOCITY; CORTEX;

D O I：

10.1117/1.3505016

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the use of spectral Doppler optical coherence tomography imaging (SDOCTI) for quantitative evaluation of dynamic blood circulation before and after a localized ischemic stroke in a mouse model. Rose Bengal photodynamic therapy (PDT) is used as a noninvasive means for inducing localized ischemia in cortical microvasculature of the mouse. Fast, repeated Doppler optical coherence tomography scans across vessels of interest are performed to record flow dynamic information with high temporal resolution. Doppler-angle-independent flow indices are used to quantify vascular conditions before and after the induced ischemia by the photocoagulation of PDT. The higher (or lower) flow resistive indices are associated with higher (or lower) resistance states that are confirmed by laser speckle flow index maps (of laser speckle imaging). Our in vivo experiments shows that SDOCTI can provide complementary quantified flow information that is an alternative to blood volume measurement, and can be used as a means for cortical microvasculature imaging well suited for small animal studies. (C) 2010 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3505016]

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