Cortical Contractility Triggers a Stochastic Switch to Fast Amoeboid Cell Motility

被引：316

作者：

Ruprecht, Verena ^{[1
]}

Wieser, Stefan ^{[1
,4
]}

Callan-Jones, Andrew ^{[5
]}

Smutny, Michael ^{[1
]}

Morita, Hitoshi ^{[1
]}

Sako, Keisuke ^{[1
]}

Barone, Vanessa ^{[1
]}

Ritsch-Marte, Monika ^{[4
]}

Sixt, Michael ^{[1
]}

Voituriez, Raphael ^{[2
,3
]}

Heisenberg, Carl-Philipp ^{[1
]}

机构：

[1] IST Austria, A-3400 Klosterneuburg, Austria

[2] Univ Paris 06, CNRS, Lab Jean Perrin, F-75005 Paris, France

[3] Univ Paris 06, CNRS, Lab Phys Theor Mat Condensee, F-75005 Paris, France

[4] Med Univ Innsbruck, Div Biomed Phys, A-6020 Innsbruck, Austria

[5] Univ Paris Diderot, CNRS, Lab Mat & Syst Complexes, UMR 7057, F-75204 Paris 13, France

来源：

CELL | 2015年 / 160卷 / 04期

基金：

奥地利科学基金会;

关键词：

LYSOPHOSPHATIDIC ACID; MIGRATION; TENSION; INVASION; REVEAL;

D O I：

10.1016/j.cell.2015.01.008

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

3D amoeboid cell migration is central to many developmental and disease-related processes such as cancer metastasis. Here, we identify a unique prototypic amoeboid cell migration mode in early zebrafish embryos, termed stable-bleb migration. Stable-bleb cells display an invariant polarized balloon-like shape with exceptional migration speed and persistence. Progenitor cells can be reversibly transformed into stable-bleb cells irrespective of their primary fate and motile characteristics by increasing myosin II activity through biochemical or mechanical stimuli. Using a combination of theory and experiments, we show that, in stable-bleb cells, cortical contractility fluctuations trigger a stochastic switch into amoeboid motility, and a positive feedback between cortical flows and gradients in contractility maintains stable-bleb cell polarization. We further show that rearward cortical flows drive stable-bleb cell migration in various adhesive and non-adhesive environments, unraveling a highly versatile amoeboid migration phenotype.

引用

页码：673 / 685

页数：13

共 30 条

[1] Forces Driving Epithelial Spreading in Zebrafish Gastrulation [J].