Genetic Dissection of Basal Resistance to Pseudomonas syringae pv. phaseolicola in Accessions of Arabidopsis

被引：47

作者：

Forsyth, Alec ^{[1
]}

Mansfield, John W. ^{[1
]}

Grabov, Nina ^{[1
]}

de Torres, Marta ^{[2
]}

Sinapidou, Eva ^{[3
]}

Grant, Murray R. ^{[2
]}

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Dept Biol, London SW7 2AZ, England

[2] Univ Exeter, Sch Biosci, Exeter EX4 4QD, Devon, England

[3] Inst Plant Protect, NAGREF, Thessaloniki 57001, Greece

来源：

MOLECULAR PLANT-MICROBE INTERACTIONS | 2010年 / 23卷 / 12期

基金：

英国生物技术与生命科学研究理事会;

关键词：

DISEASE RESISTANCE; NATURAL VARIATION; THALIANA ACCESSIONS; NONHOST RESISTANCE; INNATE IMMUNITY; PERCEPTION; IDENTIFICATION; AVIRULENCE; VIRULENCE; REVEALS;

D O I：

10.1094/MPMI-02-10-0047

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have examined the genetics of nonhost resistance in Arabidopsis, using the bean pathogen Pseudomonas syringae pv phaseolicola race 6 1448A to probe accessions for natural variation in basal defense Symptoms rarely developed in leaves of Niedersenz (Nd), some yellowing and occasional necrosis developed in Columbia (Col), whereas tissue collapse was observed in Wassilewskija (Ws) after inoculation by infiltration Analysis of F2 progeny and recombinant Inbred lines (RIL) from a cross between Col and Nd revealed a pattern of continuous symptom increase, indicating the operation of quantitative determinants of resistance By mapping quantitative trait loci (QTL), significant linkage was determined for resistance (low symptom score) to markers on chromosome 4 Segregation in the F2 cross from Nd x Ws indicated the operation of two dominant genes for resistance, one of which was FLS2 encoding the flagellin receptor The requirement for FLS2 to confer resistance was confirmed by transgenic experiments, and we showed that the response to P syringae pv phaseohcola was affected by FLS2 gene dosage Using RIL, the second locus was mapped as a QTL to a large Interval on chromosome 1 Both FLS2 and the QTL on chromosome 1 were required for the highest level of resistance to bacterial colonization and symptom development in Nd

引用

页码：1545 / 1552

页数：8

共 30 条

[1]

ALFANO JR, 1996, PLANT CELL, V8, P1698

[2]

Banerjee D, 2001, GENETICS, V158, P439

[3] HRP MUTANT OF PSEUDOMONAS-SYRINGAE PV PHASEOLICOLA INDUCES CELL-WALL ALTERATIONS BUT NOT MEMBRANE DAMAGE LEADING TO THE HYPERSENSITIVE REACTION IN LETTUCE [J].

BESTWICK, CS ;

BENNETT, MH ;

MANSFIELD, JW .

PLANT PHYSIOLOGY, 1995, 108 (02) :503-516

[4]

BOCK A, 2008, CURR OPIN PLANT BIOL, V11, P396

[5] A Renaissance of Elicitors: Perception of Microbe-Associated Molecular Patterns and Danger Signals by Pattern-Recognition Receptors [J].

Boller, Thomas ;

Felix, Georg .

ANNUAL REVIEW OF PLANT BIOLOGY, 2009, 60 :379-406

[6] Arabidopsis -: a model genus for speciation [J].

Bomblies, Kirsten ;

Weigel, Detlef .

CURRENT OPINION IN GENETICS & DEVELOPMENT, 2007, 17 (06) :500-504

[7] Natural variation in ozone sensitivity among Arabidopsis thaliana accessions and its relation to stomatal conductance [J].

Brosche, Mikael ;

Merilo, Ebe ;

Mayer, Florian ;

Pechter, Priit ;

Puzorjova, Irina ;

Brader, Gunter ;

Kangasjarvi, Jaakko ;

Kollist, Hannes .

PLANT CELL AND ENVIRONMENT, 2010, 33 (06) :914-925

[8] Use of Arabidopsis recombinant inbred lines reveals a monogenic and a novel digenic resistance mechanism to Xanthomonas campestris pv campestris [J].

Buell, CR ;

Somerville, SC .

PLANT JOURNAL, 1997, 12 (01) :21-29

[9] Pseudomonas syringae type III secretion system effectors: repertoires in search of functions [J].

Cunnac, Sebastien ;

Lindeberg, Magdalen ;

Collmer, Alan .

CURRENT OPINION IN MICROBIOLOGY, 2009, 12 (01) :53-60

[10] Pseudomonas syringae effector AvrPtoB suppresses basal defence in Arabidopsis [J].

de Torres, Marta ;

Mansfield, John W. ;

Grabov, Nina ;

Brown, Ian R. ;

Ammouneh, Hassan ;

Tsiamis, George ;

Forsyth, Alec ;

Robatzek, Silke ;

Grant, Murray ;

Boch, Jens .

PLANT JOURNAL, 2006, 47 (03) :368-382

← 1 2 3 →