Diethyldithiocarbamate inhibits iNOS expression in human lens epithelial cells stimulated by IFN-gamma and LPS

被引：10
作者：
Li, DX
Wang, SL [1 ]
Ito, Y
Zhang, JH
Wu, CF
机构：
[1] Shenyang Pharmaceut Univ, Dept Pharmaceut, Coll Pharm, Shenyang 110016, Peoples R China
[2] Kinki Univ, Sch Pharm, Osaka 5778502, Japan
[3] Kinki Univ, Pharmaceut Res & Technol Inst, Osaka 5778502, Japan
来源：
ACTA PHARMACOLOGICA SINICA | 2005年 / 26卷 / 03期
关键词：
human lens; epithelial cells; inducible nitric oxide synthase; interferon gamma; lipopolysaccharide; diethyldithiocarbamate;
D O I：
10.1111/j.1745-7254.2005.00041.x
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Aim: To investigate the biological activity of human lens epithelial cells (HLEC) in producing inducible nitric oxide synthase (iNOS) and nitric oxide (NO), and to assesse the effect of diethyldithiocarbamate (DDC) on WOS mRNA levels and expression of NOS. Methods: The human lens epithelial cell line SRA 01/04 was used in this experiment. Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and Western blotting were used to detect, respectively, WOS mRNA expression and protein production. Results: A costimulation by interferon gamma (IFN-gamma) and lipopolysaccharide (LPS) was necessary for WOS expression in HLEC. The expression of iNOS was significantly reduced in a dose-dependent manner by adding DDC from 10 mu mol/L to 1 mmol/L. Conclusion: The expression of iNOS in HLEC needs co-stimulation with IFN-gamma and LPS and it is inhibited by DDC.
引用
收藏
页码：359 / 363
页数：5
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