New Approaches for the analysis of confluent cell layers with quantitative phase digital holographic microscopy

被引：3
作者：
Pohl, L. [1 ]
Kaiser, M. [2 ]
Ketelhut, S. [1 ]
Pereira, S. [2 ]
Goycoolea, F. [2 ]
Kemper, Bjoern [1 ]
机构：
[1] Univ Munster, Fac Med, Biomed Technol Ctr, Mendelstr 17, D-48149 Munster, Germany
[2] Univ Munster, Inst Plant Biol & Biotechnol, Schlossgarten 3, D-48149 Munster, Germany
来源：
THREE-DIMENSIONAL AND MULTIDIMENSIONAL MICROSCOPY: IMAGE ACQUISITION AND PROCESSING XXIII | 2016年 / 9713卷
关键词：
digital holographic microscopy; quantitative phase imaging; live cell imaging; minimally-invasive; label-free; stain-free; confluent cell layers; CONTRAST MICROSCOPY;
D O I：
10.1117/12.2212523
中图分类号：
TH742 [显微镜];
学科分类号：
摘要：
Digital holographic microscopy (DHM) enables high resolution non-destructive inspection of technical surfaces and minimally-invasive label-free live cell imaging. However, the analysis of confluent cell layers represents a challenge as quantitative DHM phase images in this case do not provide sufficient information for image segmentation, determination of the cellular dry mass or calculation of the cell thickness. We present novel strategies for the analysis of confluent cell layers with quantitative DHM phase contrast utilizing a histogram based-evaluation procedure. The applicability of our approach is illustrated by quantification of drug induced cell morphology changes and it is shown that the method is capable to quantify reliable global morphology changes of confluent cell layers.
引用
收藏
页数：6
相关论文
共 29 条
  • [1] Differential cytotoxic actions of Shiga toxin 1 and Shiga toxin 2 on microvascular and macrovascular endothelial cells
    Bauwens, Andreas
    Bielaszewska, Martina
    Kemper, Bjoern
    Langehanenberg, Patrik
    von Bally, Gert
    Reichelt, Rudolf
    Mulac, Dennis
    Humpf, Hans-Ulrich
    Friedrich, Alexander W.
    Kim, Kwang S.
    Karch, Helge
    Muething, Johannes
    [J]. THROMBOSIS AND HAEMOSTASIS, 2011, 105 (03) : 515 - 528
  • [2] Quantitative Stain-Free and Continuous Multimodal Monitoring of Wound Healing In Vitro with Digital Holographic Microscopy
    Bettenworth, Dominik
    Lenz, Philipp
    Krausewitz, Philipp
    Brueckner, Markus
    Ketelhut, Steffi
    Domagk, Dirk
    Kemper, Bjoern
    [J]. PLOS ONE, 2014, 9 (09):
  • [3] Quadriwave lateral shearing interferometry for quantitative phase microscopy of living cells
    Bon, Pierre
    Maucort, Guillaume
    Wattellier, Benoit
    Monneret, Serge
    [J]. OPTICS EXPRESS, 2009, 17 (15): : 13080 - 13094
  • [4] Parameter-optimized digital holographic microscope for high-resolution living-cell analysis
    Carl, D
    Kemper, B
    Wernicke, G
    von Bally, G
    [J]. APPLIED OPTICS, 2004, 43 (36) : 6536 - 6544
  • [5] Tomographic phase microscopy
    Choi, Wonshik
    Fang-Yen, Christopher
    Badizadegan, Kamran
    Oh, Seungeun
    Lue, Niyom
    Dasari, Ramachandra R.
    Feld, Michael S.
    [J]. NATURE METHODS, 2007, 4 (09) : 717 - 719
  • [6] Simultaneous amplitude-contrast and quantitative phase-contrast microscopy by numerical reconstruction of Fresnel off-axis holograms
    Cuche, E
    Marquet, P
    Depeursinge, C
    [J]. APPLIED OPTICS, 1999, 38 (34) : 6994 - 7001
  • [7] High-resolution three-dimensional tomographic diffractive microscopy of transparent inorganic and biological samples
    Debailleul, M.
    Georges, V.
    Simon, B.
    Morin, R.
    Haeberle, O.
    [J]. OPTICS LETTERS, 2009, 34 (01) : 79 - 81
  • [8] Instantaneous spatial light interference microscopy
    Ding, Huafeng
    Popescu, Gabriel
    [J]. OPTICS EXPRESS, 2010, 18 (02): : 1569 - 1575
  • [9] Generalized cell morphological parameters based on interferometric phase microscopy and their application to cell life cycle characterization
    Girshovitz, Pinhas
    Shaked, Natan T.
    [J]. BIOMEDICAL OPTICS EXPRESS, 2012, 3 (08): : 1757 - 1773
  • [10] Survivin, a target to modulate the radiosensitivity of Ewing's sarcoma
    Greve, B.
    Sheikh-Mounessi, F.
    Kemper, B.
    Ernst, I.
    Goette, M.
    Eich, H. T.
    [J]. STRAHLENTHERAPIE UND ONKOLOGIE, 2012, 188 (11) : 1038 - 1047
123