A comparison of RAPD versus microsatellite DNA markers in population studies of the massasauga rattlesnake

被引：36

作者：

Lougheed, SC ^{[1
]}

Gibbs, HL

Prior, KA

Weatherhead, PJ

机构：

[1] Queens Univ, Dept Biol, Kingston, ON K7L 3N6, Canada

[2] McMaster Univ, Dept Biol, Hamilton, ON, Canada

[3] Canadian Wildlife Serv, Endangered Species Div, Hull, PQ, Canada

[4] Carleton Univ, Dept Biol, Ottawa, ON K1S 5B6, Canada

来源：

JOURNAL OF HEREDITY | 2000年 / 91卷 / 06期

基金：

加拿大自然科学与工程研究理事会;

关键词：

D O I：

10.1093/jhered/91.6.458

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We compared genetic differentiation among populations of the threatened massasauga rattlesnake (Sistrurus c. catenatus) using two types of nuclear molecular markers: randomly amplified polymorphic DNA (RAPD) markers and microsatellites. Analyses of molecular variance (AMOVA) and G(ST) and F-ST analyses Indicated that levels of among-population differentiation between regional populations (>100 km) were comparable for both markers. However, microsatellites were superior in population assignment tests and at discerning fine-scale genetic differentiation between subpopulations separated by tens of kilometers. These results argue that both types of markers are suitable for defining broad-scale genetic structures in snake populations and can provide important inputs into conservation Initiatives of focal taxa. However, our analyses suggest that microsatellites are better for detecting structure at limited spatial scales.

引用

页码：458 / 463

页数：6

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