Immunogenic properties of peptides mimicking a HIV-1 gp41 epitope recognized by virus-neutralizing antibodies 2F5

被引：2
|
作者：
Tumanova, OY [1 ]
Kuvshinov, VN
Orlovskaya, IA
Pronyaeva, TR
Pokrovskii, AG
Il'ichev, AA
Sandakhchiev, LS
机构：
[1] Vector State Res Ctr Virol & Biotechnol, Inst Bioengn, Koltsov 630559, Novosibirsk Reg, Russia
[2] Russian Acad Med Sci, Siberian Div, Inst Clin Immunol, Novosibirsk 630091, Russia
[3] Vector State Res Ctr Virol & Biotechnol, Inst Mol Biol, Koltsov 630559, Novosibirsk Reg, Russia
来源：
MOLECULAR BIOLOGY | 2003年 / 37卷 / 03期
关键词：
HIV-1; neutralizing epitope of gp41; mimicking peptides; phage display; immunogenicity; neutralizing activity;
D O I：
10.1023/A:1024259915720
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Phage display was used to obtain peptides mimicking a HIV-1 gp41 conserved epitope recognized by virus-neutralizing monoclonal antibodies (mAb) 2F5. Rabbits and mice were immunized with the peptides exposed on the surface of filamentous bacteriophages. Antibodies to gp41 were detected in the sera of immunized animals. The virus-neutralizing activity of the sera was examined.
引用
收藏
页码：473 / 476
页数：4
相关论文
共 50 条
  • [41] Heterologous Epitope-Scaffold Prime:Boosting Immuno-Focuses B Cell Responses to the HIV-1 gp41 2F5 Neutralization Determinant
    Guenaga, Javier
    Dosenovic, Pia
    Ofek, Gilad
    Baker, David
    Schief, William R.
    Kwong, Peter D.
    Hedestam, Gunilla B. Karlsson
    Wyatt, Richard T.
    PLOS ONE, 2011, 6 (01):
  • [42] Common Tolerance Mechanisms, but Distinct Cross-Reactivities Associated with gp41 and Lipids, Limit Production of HIV-1 Broad Neutralizing Antibodies 2F5 and 4E10
    Chen, Yao
    Zhang, Jinsong
    Hwang, Kwan-Ki
    Bouton-Verville, Hilary
    Xia, Shi-Mao
    Newman, Amanda
    Ouyang, Ying-Bin
    Haynes, Barton F.
    Verkoczy, Laurent
    JOURNAL OF IMMUNOLOGY, 2013, 191 (03): : 1260 - 1275
  • [43] PEPTIDES MIMICKING SELECTED DISULFIDE LOOPS IN HIV-1 GP120, OTHER THAN V3, DO NOT ELICIT VIRUS-NEUTRALIZING ANTIBODIES
    NEURATH, AR
    STRICK, N
    FIELDS, R
    JIANG, SB
    AIDS RESEARCH AND HUMAN RETROVIRUSES, 1991, 7 (08) : 657 - 662
  • [44] Structure and mechanistic analysis of the anti-human immunodeficiency virus type 1 antibody 2F5 in complex with its gp41 epitope
    Ofek, G
    Tang, M
    Sambor, A
    Katinger, H
    Mascola, JR
    Wyatt, R
    Kwong, PD
    JOURNAL OF VIROLOGY, 2004, 78 (19) : 10724 - 10737
  • [45] Retraction Note: eliciting broadly neutralizing antibodies against HIV-1 that target gp41 MPER
    D Han
    H Habte
    Y Qin
    K Takamoto
    C LaBranche
    D Montefiori
    MW Cho
    Retrovirology, 11
  • [46] Neutralizing Antibody to HIV-1 gp41 MPER Alters Recognition Properties of the Infectious Spike
    Kim, A.
    Leaman, D.
    Zwick, M.
    AIDS RESEARCH AND HUMAN RETROVIRUSES, 2013, 29 (11) : A165 - A165
  • [47] Insertion of the HIV-1 gp41 epitopes 2F5 and 4E10 into the membrane-proximal region of the vesicular stomatitis virus glycoprotein
    Lorenz, I. C.
    Nguyen, H. T.
    Phogat, S. K.
    Kaminsky, S. M.
    Zamb, T. J.
    Parks, C. L.
    RETROVIROLOGY, 2009, 6
  • [48] Recognizing a dynamic HIV-1 target: a structural look at the interaction between bnAb 2F5 and varying gp41 MPER sequences
    Julien, J.
    Bryson, S.
    Hynes, R. C.
    Pai, E. F.
    RETROVIROLOGY, 2009, 6
  • [49] Mucosal antibodies induced by tandem repeat of 2F5 epitope block transcytosis of HIV-1
    Wang, Ji
    Xu, Liling
    Tong, Pei
    Chen, Ying-Hua
    VACCINE, 2011, 29 (47) : 8542 - 8548
  • [50] Structure-guided Alterations of the gp41-directed HIV-1 Broadly Neutralizing Antibody 2F5 Reveal New Properties Regarding its Neutralizing Function
    Guenaga, Javier
    Wyatt, Richard T.
    PLOS PATHOGENS, 2012, 8 (07): : 28
12345