Crystallization and preliminary X-ray studies of a galactose-specific lectin from the seeds of bitter gourd (Momordica charantia)

被引：15

作者：

Chandran, Thyageshwar ^{[1
]}

Sharma, Alok ^{[1
]}

Vijayan, M. ^{[1
]}

机构：

[1] Indian Inst Sci, Mol Biophys Unit, Bangalore 560012, Karnataka, India

来源：

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2010年 / 66卷

关键词：

galactose-specific lectins; bitter gourd; Momordica charantia; ribosome-inactivating proteins; RIBOSOME-INACTIVATING PROTEIN; RICINUS-COMMUNIS AGGLUTININ; CRYSTAL-STRUCTURE; CARBOHYDRATE-BINDING; PURIFICATION; ENERGETICS; ABRIN;

D O I：

10.1107/S174430911002659X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A galactose-specific lectin from the seeds of bitter gourd (Momordica charantia) is a four-chain type II ribosome-inactivating protein (RIP) resulting from covalent association through a disulfide bridge between two identical copies of a two-chain unit. The available structural information on such four-chain RIPs is meagre. The bitter gourd lectin was therefore crystallized for structural investigation and the crystals have been characterized. It is anticipated that the structure of the orthorhombic crystals will be analysed using molecular replacement by taking advantage of its sequence, and presumably structural, homology to normal two-chain type II RIPs.

引用

页码：1037 / 1040

页数：4

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