Simulated microgravity promotes nitric oxide-supported angiogenesis via the iNOS-cGMP-PKG pathway in macrovascular endothelial cells

被引：40

作者：

Siamwala, Jamila H. ^{[1
]}

Majumder, Syamantak ^{[1
]}

Tamilarasan, K. P. ^{[1
]}

Muley, Ajit ^{[1
]}

Reddy, Seerapu H. ^{[1
]}

Kolluru, Gopi Krishna ^{[1
]}

Sinha, Swaraj ^{[1
]}

Chatterjee, Suvro ^{[1
]}

机构：

[1] Anna Univ, AU KBC Res Ctr, Vasc Biol Lab, Madras 600044, Tamil Nadu, India

来源：

FEBS LETTERS | 2010年 / 584卷 / 15期

关键词：

Angiogenesis; Microgravity; iNOS; Nitric oxide; Endothelial cell; GROWTH; IMPACT; MODEL;

D O I：

10.1016/j.febslet.2010.06.039

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Angiogenesis is a physiological process involving the growth of blood vessel in response to specific stimuli. The present study shows that limited microgravity treatments induce angiogenesis by activating macrovascular endothelial cells. Inhibition of nitric oxide production using pharmacological inhibitors and inducible nitric oxide synthase (iNOS) small interfering ribo nucleic acid (siRNA) abrogated microgravity induced nitric oxide production in macrovascular cells. The study further delineates that iNOS acts as a molecular switch for the heterogeneous effects of microgravity on macrovascular, endocardial and microvascular endothelial cells. Further dissection of nitric oxide downstream signaling confirms that simulated microgravity induces angiogenesis via the cyclic guanosine monophosphate (cGMP)-PKG dependent pathway. (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：3415 / 3423

页数：9

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