Complex Assembly and Metabolic Profiling of Arabidopsis thaliana Plants Overexpressing Vitamin B6 Biosynthesis Proteins

被引：27
作者：
Leuendorf, Jan Erik [2 ]
Osorio, Sonia [3 ]
Szewczyk, Agnieszka [4 ]
Fernie, Alisdair R. [3 ]
Hellmann, Hanjo [1 ]
机构：
[1] Washington State Univ, Sch Biol Sci, Pullman, WA 99164 USA
[2] Free Univ Berlin, D-14195 Berlin, Germany
[3] Max Planck Inst Mol Pflanzenphysiol, D-14476 Potsdam, Germany
[4] Jagiellonian Univ, Pharmaceut Fac, Coll Med, Krakow, Poland
来源：
MOLECULAR PLANT | 2010年 / 3卷 / 05期
关键词：
Abiotic; environmental stress; metabolomics; metabolic regulation; molecular physiology; PYRIDOXAL KINASE; 5'-PHOSPHATE OXIDASE; SYNTHASE COMPLEX; GC-MS; STRESS; GENES; PHOTORESPIRATION; EXPRESSION; TOLERANCE; MUTANT;
D O I：
10.1093/mp/ssq041
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In plants, vitamin B-6 biosynthesis requires the activity of PDX1 and PDX2 proteins. Arabidopsis thaliana encodes for three PDX1 proteins, named PDX1.1, 1.2, and 1.3, but only one PDX2. Here, we show in planta complex assembly of PDX proteins, based on split-YFP and FPLC assays, and can demonstrate their presence in higher complexes of around 750 kDa. Metabolic profiling of plants ectopically expressing the different PDX proteins indicates a negative influence of PDX1.2 on vitamin B-6 biosynthesis and a correlation between aberrant vitamin B6 content, PDX1 gene expression, and light sensitivity specifically for PDX1.3. These findings provide first insights into in planta vitamin B-6 synthase complex assembly and new information on how the different PDX proteins affect plant metabolism.
引用
收藏
页码：890 / 903
页数：14
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共 53 条
  • [11] Structural and thermodynamic insights into the assembly of the heteromeric pyridoxal phosphate synthase from Plasmodium falciparum
    Flicker, Karlheinz
    Neuwirth, Martina
    Strohmeier, Marco
    Kappes, Barbara
    Tews, Ivo
    Macheroux, Peter
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 2007, 374 (03) : 732 - 748
  • [12] Expression of bacterial L-aspartate-α-decarboxylase in tobacco increases β-alanine and pantothenate levels and improves thermotolerance
    Fouad, WM
    Rathinasabapathi, B
    [J]. PLANT MOLECULAR BIOLOGY, 2006, 60 (04) : 495 - 505
  • [13] Improper excess light energy dissipation in Arabidopsis results in a metabolic reprogramming
    Frenkel, Martin
    Kulheim, Carsten
    Jankanpaa, Hanna Johansson
    Skogstrom, Oskar
    Dall'Osto, Luca
    Agren, Jon
    Bassi, Roberto
    Moritz, Thomas
    Moen, Jon
    Jansson, Stefan
    [J]. BMC PLANT BIOLOGY, 2009, 9
  • [14] Vitamer levels, stress response, enzyme activity, and gene regulation of Arabidopsis lines mutant in the pyridoxine/pyridoxamine 5′-phosphate oxidase (PDX3) and the pyridoxal kinase (SOS4) genes involved in the vitamin B6 salvage pathway
    Gonzalez, Eugenia
    Danehower, David
    Daub, Margaret E.
    [J]. PLANT PHYSIOLOGY, 2007, 145 (03) : 985 - 996
  • [15] Vitamin B6 deficient plants display increased sensitivity to high light and photo-oxidative stress
    Havaux, Michel
    Ksas, Brigitte
    Szewczyk, Agnieszka
    Rumeau, Dominique
    Franck, Fabrice
    Caffarri, Stefano
    Triantaphylides, Christian
    [J]. BMC PLANT BIOLOGY, 2009, 9
  • [16] Vitamin B6: A Molecule for Human Health?
    Hellmann, Hanjo
    Mooney, Sutton
    [J]. MOLECULES, 2010, 15 (01): : 442 - 459
  • [17] Genetic manipulation of Vitamin B-6 biosynthesis in tobacco and fungi uncovers limitations to up-regulation of the pathway
    Herrero, Sonia
    Daub, Margaret E.
    [J]. PLANT SCIENCE, 2007, 172 (03) : 609 - 620
  • [18] Enhanced tolerance to salt stress in transgenic rice that overexpresses chloroplast glutamine synthetase
    Hoshida, H
    Tanaka, Y
    Hibino, T
    Hayashi, Y
    Tanaka, A
    Takabe, T
    Takabe, T
    [J]. PLANT MOLECULAR BIOLOGY, 2000, 43 (01) : 103 - 111
  • [19] Glutamate: Glyoxylate aminotransferase modulates amino acid content during photorespiration
    Igarashi, Daisuke
    Tsuchida, Hiroko
    Miyao, Mitsue
    Ohsumi, Chieko
    [J]. PLANT PHYSIOLOGY, 2006, 142 (03) : 901 - 910
  • [20] GMD@CSB.DB:: the Golm Metabolome Database
    Kopka, J
    Schauer, N
    Krueger, S
    Birkemeyer, C
    Usadel, B
    Bergmüller, E
    Dörmann, P
    Weckwerth, W
    Gibon, Y
    Stitt, M
    Willmitzer, L
    Fernie, AR
    Steinhauser, D
    [J]. BIOINFORMATICS, 2005, 21 (08) : 1635 - 1638
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