Cellular and humoral immune responses to mycobacterial stress proteins in experimental pulmonary tuberculosis

被引:8
|
作者
Bartow, RA [1 ]
McMurray, DN [1 ]
机构
[1] Texas A&M Univ, Coll Med, Ctr Hlth Sci, Dept Med Microbiol & Immunol, College Stn, TX 77843 USA
来源
TUBERCLE AND LUNG DISEASE | 1997年 / 78卷 / 3-4期
关键词
D O I
10.1016/S0962-8479(97)90025-3
中图分类号
R56 [呼吸系及胸部疾病];
学科分类号
摘要
Objective. Immunity to mycobacterial stress protein antigens was studied in response to vaccination and/or virulent infection. Design: Guinea pigs, either vaccinated with Mycobacterium bovis bacille Calmette-Guerin (BCG), infected by the pulmonary route with virulent M. tuberculosis, or vaccinated then infected, were studied for the development of cellular and humoral immunity to two recombinant mycobacterial stress proteins, hsp 65 and hsp 70, Results: Recombinant hsp 70 stimulated good proliferation in blood lymphocytes and, to a lesser extent, spleen and bronchotracheal lymph node lymphocytes from BCG-vaccinated guinea pigs. The proliferative responses to hsp 70 were diminished in both the spleen and lymph node cells following subsequent pulmonary challenge alone, but were boosted significantly by prior vaccination. Recombinant hsp 65 was much less active at inducing the proliferation of spleen and lymph node cells, with lowest responses observed in blood lymphocytes occurring in the cells from BCG-vaccinated, aerosol-challenged guinea pigs, Using a semi-quantitative dot blot procedure, serum antibodies to both hsp 65 and hsp 70 developed gradually following BCG vaccination, with all guinea pigs studied exhibiting significant seroreactivity after 15 weeks post-vaccination. In guinea pigs exposed to virulent M. tuberculosis by aerosol, serologic reactivity to hsp 70 was consistently stronger 6 weeks post-challenge in both vaccinated and non-vaccinated guinea pigs. In fact, 6 weeks following pulmonary exposure to M. tuberculosis in previously naive guinea pigs, 3 out of 6 animals had no detectable serum antibodies to hsp 65. Somewhat surprisingly, antibody levels to both hsp 65 and hsp 70 were only slightly increased by prior BCG vaccination in guinea pigs exposed to virulent M. tuberculosis by the respiratory route. Conclusion: These results demonstrate that both hsp 65 and hsp 70 stimulate detectable humoral and cell-mediated immunity in guinea pigs vaccinated and/or infected under highly relevant conditions. There is little evidence that vaccination with BCG primes the guinea pig to make an anamnestic response to hsp 65 following virulent pulmonary challenge. The precise contribution of immunity to mycobacterial stress proteins to the pathogenesis of tuberculosis in this model remains to be elucidated.
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收藏
页码:185 / 193
页数:9
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