Effect of PUFA on embryo cryoresistance, gene expression and AMPKα phosphorylation in IVF-derived bovine embryos

被引：48
作者：
Al Darwich, Abdulrahman [1 ,2 ,3 ,4 ]
Perreau, Christine [1 ,2 ,3 ,4 ]
Petit, Marie Helene [1 ,2 ,3 ,4 ]
Papillier, Pascal [1 ,2 ,3 ,4 ]
Dupont, Joelle [1 ,2 ,3 ,4 ]
Guillaume, Daniel [1 ,2 ,3 ,4 ]
Mermillod, Pascal [1 ,2 ,3 ,4 ]
Guignot, Florence [1 ,2 ,3 ,4 ]
机构：
[1] INRA, UMR85 Physiol Reprod & Comportements, F-37380 Nouzilly, France
[2] CNRS, UMR6175 Physiol Reprod & Comportments, F-37380 Nouzilly, France
[3] Univ Tours, F-37041 Tours, France
[4] Haras Nationaux, F-37380 Nouzilly, France
来源：
PROSTAGLANDINS & OTHER LIPID MEDIATORS | 2010年 / 93卷 / 1-2期
关键词：
Embryo culture environment; PUFA; SCD1; FADS2; SREBP1; POLYUNSATURATED FATTY-ACIDS; TRANS-10; CIS-12; ISOMER; BETA-MERCAPTOETHANOL; BLASTOCYSTS; CULTURE; STEROL; SUPPLEMENTATION; TRANSCRIPTION; ACCUMULATION; SENSITIVITY;
D O I：
10.1016/j.prostaglandins.2010.06.002
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The objectives of the present study were to evaluate the effect of conjugated linoleic acid (CLA t10, c12, C18.2). linolenic acid (C18:3) and docosahexaenoic acid (DHA. C22.6) supplementation on in vitro bovine embryo development, embryo survival after cryopreservation, gene expression and AMPK alpha phosphorylation Control groups with modified synthetic oviduct fluid (mSOF) +/- 100 mu M beta-mercaptoethanol (beta-ME) were performed. The effects of co-culture with bovine oviduct epithelial cell (Boec) monolayers, serum supplementation and embryo development in the ewe oviduct, on gene expression were also examined. Experiments 1 and 2 a lower d 7 embryo survival was found with 100 mu M C22 6 and 100 mu M C18 2 supplementation compared to 1 mu M C22.6 and 100 mu M beta-ME supplementation (P < 0 05) C18 3 supplementation had no effect on cl 7 embryo survival, but 100 mu M C18.3 increased d 8 embryo survival compared to 100 mu M beta-ME supplementation (P < 0 05) Experiments 3 and 4 stearoyl-CoA desaturase 1 (SCD1) and sterol regulatory element-binding transcription factor 1 (SREBP1) mRNA decreased after 10 mu M C22.6 supplementation compared to all other supplementations (P < 0 05) A lower fatty acid desaturase 2 (FADS2) transcript level was found with 100 mu M C18:2. 10 mu M C22:6 and 10 mu M C18:3 supplementations compared to groups without fatty acid supplementation (P < 0.05). Acetyl-CoA-carboxylase (ACC), fatty acid synthase (FAS), adipose differentiation-related protein (ADRP). acyl-CoA synthetase long-chain family member 1 (ACSL1), diacylglycerol O-acyltransferase 1 (DGAT1), carnitin palmitoyltransferase-II (CPT-II) mRNAs expression and AMPKa phosphorylation were not modified with PUFA supplementation Experiment 5. SCD1 and FAS mRNA decrease in Boec group compared to serum supplementation, as SCD1 mRNA in ewe oviduct group (P < 0 05) In conclusion, this study showed that a PUFA supplementation with C18:2, C18 3 or C22 6 in bovine culture development for 6 days and co-culture with Boec down-regulate mRNA expression of proteins involved in lipid metabolism in d 7-8 embryo (SCD1 and FADS2 desaturases), probably through SREBP1 mRNA regulation after 10 mu M C22.6 supplementation, indicating a modification of saturated/unsaturated fatty acid balance in bovine blastocyst (C) 2010 Elsevier Inc All rights reserved.
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页码：30 / 36
页数：7
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