Effect of atenolol pre-treatment in heart damage in a model of intestinal ischemia-reperfusion

被引：6

作者：

Okada, Mieko ^{[1
]}

Reis Falcao, Luiz Fernando ^{[2
]}

Ferez, David ^{[2
]}

Martins, Jose Luiz ^{[2
]}

Errante, Paolo Ruggero ^{[3
]}

Menezes Rodrigues, Francisco Sandro ^{[3
]}

Caricati-Neto, Afonso ^{[4
]}

Marinho, Marcia ^{[5
]}

Fenelon, Guilherme ^{[6
]}

Oliveira-Junior, Itamar Souza ^{[2
,7
]}

机构：

[1] Univ Fed Sao Paulo, UNIFESP, Postgrad Program Translat Med, Sao Paulo, Brazil

[2] Univ Fed Sao Paulo, Dept Surg, Div Anesthesia Pain & Intens Med, Sao Paulo, SP, Brazil

[3] Univ Fed Sao Paulo, Postgrad Program Pharmacol, Sao Paulo, SP, Brazil

[4] Univ Fed Sao Paulo, Dept Pharmacol, Sao Paulo, SP, Brazil

[5] UNESP, Vet Med Sch, Aracatuba, SP, Brazil

[6] Univ Fed Sao Paulo, Dept Surg, Div Cardiol, Sao Paulo, SP, Brazil

[7] Univ Fed Sao Paulo, Postgrad Program Translat Med, Sao Paulo, SP, Brazil

来源：

ACTA CIRURGICA BRASILEIRA | 2017年 / 32卷 / 11期

基金：

巴西圣保罗研究基金会;

关键词：

Adrenergic Antagonists; Atenolol; Ischemia; Reperfusion; Cytokines; Oxidative Stress; Rats; ADRENOCEPTOR BLOCKER; INJURY; ISCHEMIA/REPERFUSION; CARVEDILOL; APOPTOSIS; ALPHA;

D O I：

10.1590/s0102-865020170110000008

中图分类号：

R61 [外科手术学];

学科分类号：

摘要：

Purpose: To investigate the effects of atenolol in inflammatory mediator and oxidative stress in a myocardial injury by intestinal ischemia/reperfusion in rat model. Methods: Adult Wistar male rats were randomly (n= 8), anesthetized and divided in: Sham: submitted to operation only; group SS+ IR: intravenous saline infusion following superior mesenteric artery occlusion during 60 minutes (ischemia) and open for 120 minutes (reperfusion); group AT+ IR: intravenous atenolol infusion (2 mg/kg) following superior mesenteric artery occlusion during 60 minutes (ischemia) and open for 120 minutes (reperfusion); and group AT+ I+ AT+ R: intravenous atenolol infusion following superior mesenteric artery occlusion during 60 minutes (ischemia) and in the time 45 minutes other atenolol doses were administrated and the artery was open for 120 minutes (reperfusion), all animals were submitted to muscular relaxation for mechanical ventilation. In the end of experiment the animals were euthanized and the hearts tissue were morphology analyzed by histology and malondialdehyde by ELISA, and the plasma were analyzed for tumor necrosis factor-alpha by ELISA. Results: The group SS+ IR demonstrated the higher malondialdehyde levels when compared with the atenolol treated-groups (p= 0.001) in the heart tissue. The tumor necrosis factoralpha level in plasma decrease in the treated groups when compared with SS+ IR group (p= 0.001). Histology analyses demonstrate pyknosis, edema, cellular vacuolization, presence of inflammatory infiltrate and band contraction in the heart tissue of the rats. Conclusion: Atenolol significantly reduce the degree of cardiac damage after intestinal ischemia-reperfusion.

引用

页码：964 / 972

页数：9