Typing of nitrogen-fixing Frankia strains by matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry

被引：16

作者：

Hahn, Dittmar ^{[1
]}

Mirza, Babur ^{[1
]}

Benagli, Cinzia ^{[2
]}

Vogel, Guido ^{[3
]}

Tonolla, Mauro ^{[2
,4
]}

机构：

[1] Texas State Univ, Dept Biol, San Marcos, TX 78666 USA

[2] Cantonal Inst Microbiol, CH-6500 Bellinzona, Switzerland

[3] Mabritec AG, CH-4125 Riehen, Switzerland

[4] Univ Geneva, Dept Plant Biol, Microbiol Unit, CH-1211 Geneva, Switzerland

来源：

SYSTEMATIC AND APPLIED MICROBIOLOGY | 2011年 / 34卷 / 01期

关键词：

Frankia; MALDI-TOF MS; nifH gene; GENUS FRANKIA; ROOT-NODULES; ACTINORHIZAL SYMBIOSES; PHYLOGENETIC ANALYSIS; REPETITIVE SEQUENCES; MYRICA-PENSYLVANICA; MOLECULAR PHYLOGENY; BAYESIAN-INFERENCE; ALNUS-GLUTINOSA; CASUARINA;

D O I：

10.1016/j.syapm.2010.11.009

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry (MS) was evaluated as a technique to characterize strains of the nitrogen-fixing actinomycete Frankia. MALDI-TOF MS reliably distinguished 37 isolates within the genus Frankia and assigned them to their respective host infection groups, i.e., the Alnus/Casuarina and the Elaeagnus host infection groups. The assignment of individual strains to sub-groups within the respective host infection groups was consistent with classification based on comparative sequence analysis of nifH gene fragments, confirming the usefulness of MALDI-TOF MS as a rapid and reliable tool for the characterization of Frankia strains. (C) 2010 Elsevier GmbH. All rights reserved.

引用

页码：63 / 68

页数：6

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