Unsupported planar lipid membranes formed from mycolic acids of Mycobacterium tuberculosis

被引：3

作者：

Langford, Kyle W. ^{[1
]}

Penkov, Boyan ^{[1
]}

Derrington, Ian M. ^{[1
]}

Gundlach, Jens H. ^{[1
]}

机构：

[1] Univ Washington, Dept Phys, Seattle, WA 98195 USA

来源：

JOURNAL OF LIPID RESEARCH | 2011年 / 52卷 / 02期

基金：

美国国家卫生研究院;

关键词：

mycolic acid; membrane; sequencing; MspA; nanopore; CELL-WALL; FORCE SPECTROSCOPY; OUTER-MEMBRANE; SMEGMATIS; BILAYERS; NANOPORE; PROTEINS; MSPA; NANOMECHANICS; BIOSYNTHESIS;

D O I：

10.1194/jlr.m012013

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cell wall of mycobacteria includes a thick, robust, and highly impermeable outer membrane made from long-chain mycolic acids. These outer membranes form a primary layer of protection for mycobacteria and directly contribute to the virulence of diseases such as tuberculosis and leprosy. We have formed in vitro planar membranes using pure mycolic acids on circular apertures 20 to 90 mu m in diameter. We find these membranes to be long lived and highly resistant to irreversible electroporation, demonstrating their general strength. Insertion of the outer membrane channel MspA into the membranes was observed indicating that the artificial mycolic acid membranes are suitable for controlled studies of the mycobacterial outer membrane and can be used in nanopore DNA translocation experiments.-Langford, K. W., B. Penkov, I. M. Derrington, and J. H. Gundlach. Unsupported planar lipid membranes formed from mycolic acids of Mycobacterium tuberculosis. J. Lipid Res. 2011. 52: 272-277.

引用

页码：272 / 277

页数：6

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