Metabolic engineering of Escherichia coli to produce (2S, 3R, 4S)-4-hydroxyisoleucine

被引：58

作者：

Smirnov, Sergey V. ^{[1
]}

Kodera, Tomohiro ^{[2
]}

Samsonova, Natalya N. ^{[1
]}

Kotlyarova, Veronika A. ^{[1
]}

Rushkevich, Natalya Yu ^{[1
]}

Kivero, Alexander D. ^{[1
]}

Sokolov, Pavel M. ^{[1
]}

Hibi, Makoto ^{[3
]}

Ogawa, Jun ^{[3
]}

Shimizu, Sakayu ^{[3
]}

机构：

[1] Ajinomoto Genet Res Inst, Moscow 117545, Russia

[2] Ajinomoto Co Inc, Inst Life Sci, Kawasaki Ku, Kawasaki, Kanagawa 2108681, Japan

[3] Kyoto Univ, Div Appl Life Sci, Grad Sch Agr, Sakyo Ku, Kyoto 6068502, Japan

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2010年 / 88卷 / 03期

关键词：

4-Hydroxyisoleucine; Diabetes; Dioxygenase; Biotransformation; Escherichia coli; AMINO-ACID; 4-HYDROXYISOLEUCINE; IDENTIFICATION; ALDOLASE; GENES;

D O I：

10.1007/s00253-010-2772-3

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The stereo-specific l-isoleucine-4-hydroxylase (l-isoleucine dioxygenase (IDO)) was cloned and expressed in an Escherichia coli 2 Delta strain lacking the activities of alpha-ketoglutarate dehydrogenase (EC 1.2.4.2), isocitrate liase (EC 4.1.3.1), and isocitrate dehydrogenase kinase/phosphatase (EC 2.7.11.5). The 2 Delta strain could not grow in a minimal-salt/glucose/glycerol medium due to the blockage of TCA during succinate synthesis. The IDO activity in the 2 Delta strain was able to "shunt" destroyed TCA, thereby coupling l-isoleucine hydroxylation and cell growth. Using this strain, we performed the direct biotransformation of l-isoleucine into 4-HIL with an 82% yield.

引用

页码：719 / 726

页数：8

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