Isolation of balenine from opah (Lampris megalopsis) muscle and comparison of antioxidant and iron-chelating activities with other major imidazole dipeptides

被引：12

作者：

Ishihara, Kenji ^{[1
]}

Watanabe, Ryuichi ^{[1
]}

Kato, Tomomi ^{[1
]}

Seko, Takuya ^{[1
]}

Matsuda, Takashi ^{[1
]}

Omura, Yuji ^{[2
]}

Shigemura, Yasutaka ^{[3
]}

Kawabata, Yasunosuke ^{[4
]}

Maegawa, Takahiro ^{[4
]}

机构：

[1] Japan Fisheries Res & Educ Agency, Fisheries Technol Inst, Seafood Safety & Technol Div, Yokohama, Kanagawa, Japan

[2] Japan Fisheries Res & Educ Agency, Marine Fisheries Res & Dev Ctr, Yokohama, Kanagawa, Japan

[3] Tokyo Kasei Univ Tokyo, Fac Domest Sci, Dept Nutr, Tokyo, Japan

[4] Kyokuyo Co Ltd, Shiogama Inst, Shiogama, Miyagi, Japan

来源：

FOOD CHEMISTRY | 2021年 / 364卷

关键词：

Balenine; Opah; Imidazole dipeptides; Carnosine; Anserine; Antioxidant; BETA-ALANINE SUPPLEMENTATION; CARNOSINE; EXERCISE; ANSERINE;

D O I：

10.1016/j.foodchem.2021.130343

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Balenine (Bal) in opah muscle was extracted using hot water and purified by ion-exchange chromatography and recrystallization to provide 41 g of over 95% pure Bal from 1 kg of opah muscle. The structure of purified Bal was identical to that of an authentic Bal standard by NMR analysis. The antioxidant (ORAC and HORAC values) and Fe(II) ion-chelating abilities of purified Bal were examined by comparison with two major imidazole dipeptides, carnosine (Car) and anserine (Ans). Opah-derived Bal showed significantly higher ORAC and HORAC values and Fe(II) ion-chelating ability at 0.3 mM. In silico molecular simulation revealed that Bal and Car formed hydrogen bonds between the hydrogen atom of the imidazole imino group and the carboxyl carbonyl oxygen, whereas Ans did not. The proposed method for extracting and purifying Bal from opah muscle suggests that opah can be utilized as a functional food or Bal resource.

引用

页数：6