Vitexin, Orientin and Other Flavonoids from Spirodela polyrhiza Inhibit Adipogenesis in 3T3-L1 Cells

被引：98

作者：

Kim, JinPyo ^{[1
]}

Lee, IkSoo ^{[1
]}

Seo, JeongJu ^{[2
]}

Jung, MunYhung ^{[3
]}

Kim, YoungHee ^{[4
]}

Yim, NamHui ^{[1
]}

Bae, KiHwan ^{[1
]}

机构：

[1] Chungnam Natl Univ, Coll Pharm, Taejon 305764, South Korea

[2] Kyungpook Natl Univ, Sch Med, Dept Mol Med, Taegu, South Korea

[3] Woosuk Univ, Dept Food Ind & Management, Wanju Kun 565701, Jeonbuk, South Korea

[4] Korea Res Inst Biosci & Biotechnol, Taejon 305806, South Korea

来源：

PHYTOTHERAPY RESEARCH | 2010年 / 24卷 / 10期

关键词：

vitexin; orientin; Spirodela polyrhiza; flavonoids; 3T3-L1; adipogenesis; GENE-EXPRESSION; GLUCOSE-UPTAKE; TISSUE;

D O I：

10.1002/ptr.3186

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

To investigate the adipogenesis inhibitory effect on lipid accumulation, 3T3-L1 cells were treated with fractions and isolated flavonoids of Spirodela polyrhiza. An ethanol extract of S. polyrhiza was fractionated into three fractions. The butanol soluble fraction (SPB) exhibited potent antiadipogenesis activity and decreased C/EBP alpha and PPAR. protein expression level in 3T3-L1 cells without significant cytotoxicity. The flavonoids were isolated from SPB and their chemical structures were identified as chrysoeriol (1), apigenin (2), luteolin (3), vitexin (4), cosmosin (5), orientin (6) and luteolin-7-O-beta-D-glucoside (7) by spectroscopic analysis. Studies on the adipogenesis and intracellular triglyceride accumulation inhibitory effect showed that compounds 4 and 6 had the highest activity and decreased C/EBP alpha and PPAR gamma protein expression level in 3T3-L1 cells. These results suggest that the flavonoids isolated from SPB, especially compounds 4 and 6, contribute to the inhibitory activity of S. polyrhiza in 3T3-L1 cells. Copyright (c) 2010 John Wiley & Sons, Ltd.

引用

页码：1543 / 1548

页数：6

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