Anxiolytic and neuroprotective effects of the Traditional Chinese Medicinal formulation Dan-zhi-xiao-yao-san in a rat model of chronic stress

被引：21
作者：
Cao, Guo-Ping [1 ]
Gui, Dan [1 ]
Fu, Lu-Di [1 ]
Guo, Zhou-Ke [1 ,2 ]
Fu, Wen-Jun [1 ]
机构：
[1] Guangzhou Univ Chinese Med, Dept Basic Med Sci, 232 East Waihuan Rd, Guangzhou 510006, Guangdong, Peoples R China
[2] Shenzhen Hosp Chinese Med, Dept Neurol & Psychol, 1 Fuhua Rd, Shenzhen 518033, Guangdong, Peoples R China
来源：
MOLECULAR MEDICINE REPORTS | 2016年 / 14卷 / 02期
基金：
中国国家自然科学基金;
关键词：
Dan-zhi-xiao-yao-san; anxiolytic; stress; alpha-synuclein; corticosterone; ANXIETY DISORDERS; ANIMAL-MODELS; PHARMACOLOGICAL-TREATMENT; ALPHA-SYNUCLEIN; DISEASE; BRAIN; METAANALYSIS; BEHAVIOR; HEALTH; SAFETY;
D O I：
10.3892/mmr.2016.5382
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Dan-zhi-xiao-yao-san is a Traditional Chinese Medicinal formulation widely used for the treatment of neuropsychological disorders. The present study examined the anxiolytic and neuroprotective effects of Dan-zhi-xiao-yao-san in a rat model of chronic stress. The results of an elevated plus maze test showed that Dan-zhi-xiao-yao-san significantly attenuated the levels of anxiety-induced stress as evidenced by increases in the time spent in the open arm region, as well as the percentage of entries into this area. In addition, Dan-zhi-xiao-yao-san alleviated stress-induced neuronal death, as indicated by histological examination. Furthermore, mechanistic studies suggested that the anxiolytic and neuroprotective effects of Dan-zhi-xiao-yao-san may be mediated via attenuation of chronic stress-induced upregulation of alpha-synuclein and corticosterone, and downregulation of protein phosphatase 2A (PP2A) in the hippocampal region of the brain at the mRNA and protein level. In addition, Dan-zhi-xiao-yao-san decreased the serum levels of stress-induced corticosterone in the model animals. In conclusion, the present study demonstrated that Dan-zhi-xiao-yao-san exerted anxiolytic and neuroprotective effects in a rat model of chronic stress via attenuation of stress-induced upregulation of alpha-synuclein and corticosterone, and downregulation of PP2A in the hippocampus.
引用
收藏
页码：1247 / 1254
页数：8
相关论文
共 41 条
[1]   Guidelines for the pharmacological treatment of anxiety disorders, obsessive-compulsive disorder and posttraumatic stress disorder in primary care [J].
Bandelow, Borwin ;
Sher, Leo ;
Bunevicius, Robertas ;
Hollander, Eric ;
Kasper, Siegfried ;
Zohar, Joseph ;
Moeller, Hans-Juergen .
INTERNATIONAL JOURNAL OF PSYCHIATRY IN CLINICAL PRACTICE, 2012, 16 (02) :77-84
[2]   The stress-induced hyperthermia paradigm as a physiological animal model for anxiety: A review of pharmacological and genetic studies in the mouse [J].
Bouwknecht, J. Adriaan ;
Olivier, Berend ;
Paylor, Richard E. .
NEUROSCIENCE AND BIOBEHAVIORAL REVIEWS, 2007, 31 (01) :41-59
[3]   Use of Complementary and Alternative Medicine in a Large Sample of Anxiety Patients [J].
Bystritsky, Alexander ;
Hovav, Sarit ;
Sherbourne, Cathy ;
Stein, Murray B. ;
Rose, Raphael D. ;
Campbell-Sills, Laura ;
Golinelli, Daniela ;
Sullivan, Greer ;
Craske, Michelle G. ;
Roy-Byrne, Peter P. .
PSYCHOSOMATICS, 2012, 53 (03) :266-272
[4]   Animal models of anxiety disorders and stress [J].
Campos, Alline C. ;
Fogaca, Manoela V. ;
Aguiar, Daniele C. ;
Guimaraes, Francisco S. .
REVISTA BRASILEIRA DE PSIQUIATRIA, 2013, 35 :S101-S111
[5]   Prescription patterns of Chinese herbal products for menopausal syndrome: Analysis of a nationwide prescription database [J].
Chen, Hsing-Yu ;
Lin, Yi-Hsuan ;
Wu, Jau-Ching ;
Chen, Yu-Chun ;
Yang, Sien-Hung ;
Chen, Jiun-Liang ;
Chen, Tzeng-Ji .
JOURNAL OF ETHNOPHARMACOLOGY, 2011, 137 (03) :1261-1266
[6]  
Chen Wen-Fang, 2012, J Tradit Complement Med, V2, P67
[7]   Expression of α-synuclein is increased in the hippocampus of rats with high levels of innate anxiety [J].
Chiavegatto, S. ;
Izidio, G. S. ;
Mendes-Lana, A. ;
Aneas, I. ;
Freitas, T. A. ;
Torrao, A. S. ;
Conceicao, I. M. ;
Britto, L. R. G. ;
Ramos, A. .
MOLECULAR PSYCHIATRY, 2009, 14 (09) :894-905
[8]   A Chinese Herbal Medicine, Jia-Wei-Xiao-Yao-San, Prevents Dimethylnitrosamine-Induced Hepatic Fibrosis in Rats [J].
Chien, Shu-Chen ;
Chang, Wei-Chiao ;
Lin, Pu-Hua ;
Chang, Wei-Pin ;
Hsu, Shih-Chung ;
Chang, Jung-Chen ;
Wu, Ya-Chieh ;
Pei, Jin-Kuo ;
Lin, Chia-Hsien .
SCIENTIFIC WORLD JOURNAL, 2014,
[9]   The use of mifepristone in the treatment of neuropsychiatric disorders [J].
DeBattista, C ;
Belanoff, J .
TRENDS IN ENDOCRINOLOGY AND METABOLISM, 2006, 17 (03) :117-121
[10]   Prevalence, severity, and unmet need for treatment of mental disorders in the World Health Organization World Mental Health Surveys [J].
Demyttenaere, K ;
Bruffaerts, R ;
Posada-Villa, J ;
Gasquet, I ;
Kovess, V ;
Lepine, JP ;
Angermeyer, MC ;
Bernert, S ;
de Girolamo, G ;
Morosini, P ;
Polidori, G ;
Kikkawa, T ;
Kawakami, N ;
Ono, Y ;
Takeshima, T ;
Uda, H ;
Karam, EG ;
Fayyad, JA ;
Karam, AN ;
Mneimneh, ZN ;
Medina-Mora, ME ;
Borges, G ;
Lara, C ;
de Graaf, R ;
Ormel, J ;
Gureje, O ;
Shen, YC ;
Huang, YQ ;
Zhang, MY ;
Alonso, J ;
Haro, JM ;
Vilagut, G ;
Bromet, EJ ;
Gluzman, S ;
Webb, C ;
Kessler, RC ;
Merikangas, KR ;
Anthony, JC ;
Von Korff, MR ;
Wang, PS ;
Alonso, J ;
Brugha, TS ;
Aguilar-Gaxiola, S ;
Lee, S ;
Heeringa, S ;
Pennell, BE ;
Zaslavsky, AM ;
Ustun, TB ;
Chatterji, S .
JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 2004, 291 (21) :2581-2590
12345