Metabolic model of Synechococcus sp PCC 7002: Prediction of flux distribution and network modification for enhanced biofuel production

被引：67

作者：

Hendry, John I. ^{[1
]}

Prasannan, Charulata B. ^{[1
]}

Joshi, Aditi ^{[1
]}

Dasgupta, Santanu ^{[4
]}

Wangikar, Pramod P. ^{[1
,2
,3
]}

机构：

[1] Indian Inst Technol, Dept Chem Engn, Bombay 400076, Maharashtra, India

[2] Indian Inst Technol, DBT Pan IIT Ctr Bioenergy, Bombay 400076, Maharashtra, India

[3] Indian Inst Technol, Wadhwani Res Ctr Bioengn, Bombay 400076, Maharashtra, India

[4] Reliance Ind Ltd, Reliance Technol Grp, Reliance Corp Pk, Ghansoli 400701, Navi Mumbai, India

来源：

BIORESOURCE TECHNOLOGY | 2016年 / 213卷

关键词：

Cyanobacteria; Synechococcus sp PCC 7002; Genome scale metabolic model; Flux Balance Analysis; Minimization of Metabolic Adjustments (MOMA); CYANOBACTERIA; CAROTENOIDS; OPTIMALITY; GENES;

D O I：

10.1016/j.biortech.2016.02.128

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

Flux Balance Analysis was performed with the Genome Scale Metabolic Model of a fast growing cyanobacterium Synechococcus sp. PCC 7002 to gain insights that would help in engineering the organism as a production host. Gene essentiality and synthetic lethality analysis revealed a reduced metabolic robustness under genetic perturbation compared to the heterotrophic bacteria Escherichia coli. Under glycerol heterotrophy the reducing equivalents were generated from tricarboxylic acid cycle rather than the oxidative pentose phosphate pathway. During mixotrophic growth in glycerol the photosynthetic electron transport chain was predominantly used for ATP synthesis with a photosystem I/photosystem II flux ratio higher than that observed under autotrophy. An exhaustive analysis of all possible double reaction knock outs was performed to reroute fixed carbon towards ethanol and butanol production. It was predicted that only similar to 10% of fixed carbon could be diverted for ethanol and butanol production. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：190 / 197

页数：8

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