Mast Cell Infiltration Is Involved in Renal Interstitial Fibrosis in a Rat Model of Protein-Overload Nephropathy

被引：16

作者：

Li, Ying ^{[1
]}

Zhou, Letian ^{[1
]}

Liu, Fuyou ^{[1
]}

Peng, Youming ^{[1
]}

Li, Jun ^{[1
]}

Sun, Lin ^{[1
]}

Duan, Shaobin ^{[1
]}

Ling, Guanghui ^{[1
]}

Chen, Xin ^{[1
]}

Jiang, Wenling ^{[1
]}

Xia, Yuncheng ^{[1
]}

机构：

[1] Cent South Univ, Key Lab Nephrol & Blood Purificat Hunan, Res Inst Nephrol, Div Nephrol,Xiangya Hosp 2, Changsha 410011, Hunan, Peoples R China

来源：

KIDNEY & BLOOD PRESSURE RESEARCH | 2010年 / 33卷 / 03期

关键词：

Protein-overload nephropathy; Mast cell; Stem cell factor; Transforming growth factor-beta(1); Renal interstitial fibrosis; TUBULOINTERSTITIAL FIBROSIS; TGF-BETA-1; INFLAMMATION; EXPRESSION; KIDNEY; MACROPHAGES; PROGRESSION; DAMAGE;

D O I：

10.1159/000317102

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

Aims: The present study investigated the relationship between mast cells (MCs) and the protein expression of stem cell factor (SCF) and transforming growth factor-beta(1) (TGF-beta(1)) in the regions of renal interstitial fibrosis with protein-overload nephropathy, in order to provide a good animal model to study the mechanism of renal fibrosis induced by proteinuria. Methods: 60 male Sprague-Dawley rats were divided into a bovine serum albumin (BSA) group and a control group. The intensity of MCs infiltration was examined by toluidine blue and chymase and tryptase staining. The protein expression of SCF and TGF-beta(1) was respectively examined by immunohistochemical and immunofluorescence staining. Results: Severe proteinuria was induced in rats of the BSA group. Expression of SCF and TGF-beta(1) was detected in the tubular and the interstitial cells. The number of MCs positively correlated with the severity of interstitial lesions and the expression of SCF and TGF-beta(1). Conclusion: Our results demonstrated that in protein-overload nephropathy, MCs infiltrated into the kidney, and the expression of SCF and TGF-beta(1) gradually increased. They might play important roles in the development of renal interstitial fibrosis, but the underlying mechanism needs to be further studied. Copyright (C) 2010 S. Karger AG, Basel

引用

页码：240 / 248

页数：9