Specific nanotoxicity of graphene oxide during zebrafish embryogenesis

被引:154
作者
Chen, Yuming [1 ]
Hu, Xiangang [1 ]
Sun, Jing [1 ]
Zhou, Qixing [1 ]
机构
[1] Nankai Univ, Coll Environm Sci & Engn, Tianjin Key Lab Environm Remediat & Pollut Contro, Key Lab Pollut Proc & Environm Criteria,Minist Ed, Tianjin 300071, Peoples R China
基金
中国国家自然科学基金;
关键词
Hatching delay; malformation; mitochondrial dysfunction; oxidative stress; uptake; FISH CELL-LINE; IN-VIVO; QUANTUM DOTS; SILVER NANOPARTICLES; TOXICITY; NANOMATERIALS; CYTOTOXICITY; NANOSHEETS; BIOCOMPATIBILITY; METHYLATION;
D O I
10.3109/17435390.2015.1005032
中图分类号
TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
Graphene oxide (GO) has shown great potential for biological, medical, energy and electronic applications. As a consequence of these diverse applications, GO release into the ecosystem is inevitable; however, the corresponding risks are largely unknown, particularly with respect to the critical period of embryogenesis. This study revealed that GO adhered to and enveloped the chorion of zebrafish embryos mainly via hydroxyl group interactions, blocked the pore canals of the chorionic membrane, and caused marked hypoxia and hatching delay. Furthermore, GO spontaneously penetrated the chorion, entered the embryo via endocytosis, damaged the mitochondria and primarily translocated to the eye, heart and yolk sac regions, which are involved in the circulatory system of zebrafish. In these organs, GO induced excessive generation of reactive oxygen species and increased oxidative stress, DNA damage and apoptosis. Graphene oxide also induced developmental malformation of the eye, cardiac/yolk sac edema, tail flexure and heart rate reduction. In contrast to the common dose-effect relationships of nanoparticles, the adverse effects of GO on heart rate and tail/spinal cord flexure increased and then decreased as the GO concentration increased. These findings emphasize the specific adverse effects of GO on embryogenesis and highlight the potential ecological and health risks of GO.
引用
收藏
页码:42 / 52
页数:11
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