Quantitative Cryo-Electron Tomography

被引：10

作者：

Navarro, Paula P. ^{[1
,2
]}

机构：

[1] Massachusetts Gen Hosp, Dept Mol Biol, Boston, MA 02114 USA

[2] Harvard Med Sch, Dept Genet, Boston, MA 02115 USA

来源：

FRONTIERS IN MOLECULAR BIOSCIENCES | 2022年 / 9卷

关键词：

cryo-electron tomography; in situ structural biology; quantitative cryo-electron tomography; image processing; cell biology; IN-SITU; MACROMOLECULAR COMPLEXES; CRYO-EM; ELECTRON TOMOGRAPHY; MOLECULAR SOCIOLOGY; MICROSCOPY; FROZEN; CELLS; RECONSTRUCTION; INFORMATION;

D O I：

10.3389/fmolb.2022.934465

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The three-dimensional organization of biomolecules important for the functioning of all living systems can be determined by cryo-electron tomography imaging under native biological contexts. Cryo-electron tomography is continually expanding and evolving, and the development of new methods that use the latest technology for sample thinning is enabling the visualization of ever larger and more complex biological systems, allowing imaging across scales. Quantitative cryo-electron tomography possesses the capability of visualizing the impact of molecular and environmental perturbations in subcellular structure and function to understand fundamental biological processes. This review provides an overview of current hardware and software developments that allow quantitative cryo-electron tomography studies and their limitations and how overcoming them may allow us to unleash the full power of cryo-electron tomography.

引用

页数：11

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