Promotion of chondrogenic differentiation of mesenchymal stem cells by copper: Implications for new cartilage repair biomaterials

被引:36
作者
Xu, Changkui [1 ,2 ,3 ]
Chen, Jiarong [3 ]
Li, Lihua [3 ]
Pu, Xiaobing [4 ]
Chu, Xiao [3 ]
Wang, Xiaolan [3 ]
Li, Mei [3 ]
Lu, Yao [3 ,5 ]
Zheng, Xiaofei [1 ]
机构
[1] Guangzhou Overseas Chinese Hosp, Dept Orthopaed, 613 West Whampoa Rd, Guangzhou 510630, Guangdong, Peoples R China
[2] Foshan Saushui Dist Peoples Hosp, Dept Orthopaed, 16 West Guanghai Rd, Foshan 528100, Guangdong, Peoples R China
[3] Guangzhou Mil Command PLA, Guangdong Key Lab Orthopaed Technol & Implant Mat, Key Lab Trauma & Tissue Repair Trop Area PLA, Dept Orthopaed,Gen Hosp, Guangzhou 510010, Guangdong, Peoples R China
[4] Sichuan Univ, West China Teaching Hosp 4, Dept Orthopaed, 18 South Rennin Rd, Chengdu 60041, Sichuan, Peoples R China
[5] Southern Med Univ, Zhujiang Hosp, Dept Orthopaed, 253 Gongye Rd, Guangzhou 510282, Guangdong, Peoples R China
来源
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS | 2018年 / 93卷
基金
中国国家自然科学基金;
关键词
Copper; Mesenchymal stem cells; Chondrogenic differentiation; Cartilage repair; IN-VITRO CHONDROGENESIS; BONE; SCAFFOLD; DEFECTS; MATRIX; MICROFRACTURE; REGENERATION; CHONDROCYTES; TRANSPORT; HYDROGELS;
D O I
10.1016/j.msec.2018.07.074
中图分类号
TB3 [工程材料学]; R318.08 [生物材料学];
学科分类号
0805 ; 080501 ; 080502 ;
摘要
Copper (Cu) has drawn considerable attention in the design of biomaterials due to its multifunction, such as antibacterial property, osteogenic and angiogenic ability. However, the effect of Cu on chondrogenic differentiation of mesenchymal stem cells (MSCs) and its potential for cartilage repair biomaterials has been rarely studied. Here, we report that Cu can significantly enhance chondrogensis of MSCs. Specifically, in vitro studies showed that Cu could promote MSCs cytoskeleton change, extracellular glycosaminoglycan (GAG) deposition and the chrodrogenic genes (Sox9, Aggrecan, and Col-2) up-regulation. Furthermore, we prepared a Cu-containing alginate (Alg) porous scaffold to assess the chondroinductivity of Cu in vivo. In eight weeks, we found that Alg/Cu scaffolds could induce better formation of new cartilage tissue compared to the pure Alg scaffolds fabricated by the same procedure but without adding Cu. These encouraging results indicate that Cu can bring considerable benefits to the development and application of cartilage repair biomaterials.
引用
收藏
页码:106 / 114
页数:9
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