Genetic Differentiation, Diversity, and Drug Susceptibility of Candida krusei

被引：21

作者：

Gong, Jie ^{[1
]}

Xiao, Meng ^{[2
,3
]}

Wang, He ^{[2
,3
]}

Kudinha, Timothy ^{[4
,5
]}

Wang, Yu ^{[6
]}

Zhao, Fei ^{[1
]}

Wu, Weiwei ^{[7
]}

He, Lihua ^{[1
]}

Xu, Ying-Chun ^{[2
,3
]}

Zhang, Jianzhong ^{[1
]}

机构：

[1] Chinese Ctr Dis Control & Prevent, Natl Inst Communicable Dis Control & Prevent, Collaborat Innovat Ctr Diag & Treatment Infect Di, State Key Lab Infect Dis Prevent & Control, Beijing, Peoples R China

[2] Chinese Acad Med Sci, Peking Union Med Coll Hosp, Dept Clin Lab, Beijing, Peoples R China

[3] Beijing Key Lab Mech Res & Precis Diag Invas Fung, Beijing, Peoples R China

[4] Charles Sturt Univ, Sch Biomed Sci, Orange, NSW, Australia

[5] Cent West Pathol Lab, Orange, NSW, Australia

[6] Zhejiang Normal Univ, Key Lab Wildlife Biotechnol Conservat & Utilizat, Jinhua, Peoples R China

[7] Hainan Prov Ctr Skin Dis & STI Control, Dept Dermatol, Haikou, Hainan, Peoples R China

来源：

FRONTIERS IN MICROBIOLOGY | 2018年 / 9卷

关键词：

Candida krusei; invasive candidiasis; genetic differentiation; genetic diversity; microsatellites; drug susceptibility; POPULATION-STRUCTURE; RESISTANCE; VORICONAZOLE; SOFTWARE; MICROSATELLITE; CASPOFUNGIN; FLUCONAZOLE; EMERGENCE; PATHOGEN; SEQUENCE;

D O I：

10.3389/fmicb.2018.02717

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Candida krusei is a notable pathogenic fungus that causes invasive candidiasis, mainly due to its natural resistance to fluconazole. However, to date, there is limited research on the genetic population features of C. krusei. We developed a set of microsatellite markers for this organism, with a cumulative discriminatory power of 1,000. Using these microsatellite loci, 48 independent C. krusei strains of clearly known the sources, were analyzed. Furthermore, susceptibility to 9 antifungal agents was determined for each strain, by the Clinical and Laboratory Standards Institute broth microdilution method. Population structure analyses revealed that C. krusei could be separated into two clusters. The cluster with the higher genetic diversity had wider MIC ranges for six antifungal agents. Furthermore, the highest MIC values of the six antifungal agents belonged to the cluster with higher genetic diversity. The higher genetic diversity cluster might have a better adaptive capacity when C. krusei is under selection pressure from antifungal agents, and thus is more likely to develop drug resistance.

引用

页数：10

共 35 条

[1] EMERGENCE OF CANDIDA-KRUSEI INFECTIONS AFTER THERAPY OF OROPHARYNGEAL CANDIDIASIS WITH FLUCONAZOLE [J].