Simultaneous determination of isothiocyanates, indoles, and oxazolidinethiones in myrosinase digests of rapeseeds and rapeseed meal by HPLC

HPLC has been used for the analysis of isothiocyanates, indoles, and oxazolidinethiones in rapeseeds and rapeseed meal. The samples were treated with myrosinase and the released hydrolysis products extracted with dichloromethane. The separation was performed on an RP-18 column using a gradient system with acetonitrile and water. Use of a programmable UV detector permitted the detection of the compounds at their absorption maxima of 210 and 240 nm, respectively. Response factors of eight standard compounds were calculated for 240 nm. The contents of glucosinolates calculated with the results of this method showed a significant linear correlation (r = 0.9995; P < 0.005) with the contents of glucosinolates evaluated with the results of the HPLC method of desulfoglucosinolates.