Diffusible and Volatile Antifungal Compounds Produced by an Antagonistic Bacillus velezensis G341 against Various Phytopathogenic Fungi

被引：142
作者：
Lim, Seong Mi [1 ]
Yoon, Mi-Young [2 ]
Choi, Gyung Ja [2 ]
Choi, Yong Ho [2 ]
Jang, Kyoung Soo [2 ]
Shin, Teak Soo [3 ]
Park, Hae Woong [4 ]
Yu, Nan Hee [1 ]
Kim, Young Ho [5 ,6 ]
Kim, Jin-Cheol [1 ]
机构：
[1] Chonnam Natl Univ, Inst Environm Friendly Agr, Div Appl Biosci & Biotechnol, Coll Agr & Life Sci, Gwangju 61186, South Korea
[2] Korea Res Inst Chem Technol, Ecofriendly New Mat Res Grp, Daejeon 34114, South Korea
[3] Farm Hannong Co Ltd, Crop Protect Res Ctr, Chungnam 33010, South Korea
[4] World Inst Kimchi, R&D Div, Gwangju 61755, South Korea
[5] Seoul Natl Univ, Dept Agr Biotechnol, Seoul 08826, South Korea
[6] Seoul Natl Univ, Res Inst Agr & Life Sci, Seoul 08826, South Korea
来源：
PLANT PATHOLOGY JOURNAL | 2017年 / 33卷 / 05期
关键词：
antifungal metabolite; Bacillus velezensis; biocontrol; phytopathogenic fungi; PLANT-PATHOGENIC FUNGI; BIOLOGICAL-CONTROL; SYSTEMIC RESISTANCE; BIOCONTROL ACTIVITY; RHIZOCTONIA-SOLANI; BOTRYTIS-CINEREA; SUBTILIS F-29-3; POWDERY MILDEW; BACILLOMYCIN-D; ITURIN GROUP;
D O I：
10.5423/PPJ.OA.04.2017.0073
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
The aim of this study was to identify volatile and agar diffusible antifungal metabolites produced by Bacillus sp. G341 with strong antifungal activity against various phytopathogenic fungi. Strain G341 isolated from fouryear -old roots of Korean ginseng with rot symptoms was identified as Bacillus velezensis based on 16S rDNA and gyrA sequences. Strain G341 inhibited mycelial growth of all phytopathogenic fungi tested. In vivo experiment results revealed that n-butanol extract of fermentation broth effectively controlled the development of rice sheath blight, tomato gray mold, tomato late blight, wheat leaf rust, barley powdery mildew, and red pepper anthracnose. Two antifungal compounds were isolated from strain G341 and identified as bacillomycin L and fengycin A by MS/MS analysis. Moreover, volatile compounds emitted from strain G341 were found to be able to inhibit mycelial growth of various phytopathogenic fungi. Based on volatile compound profiles of strain G341 obtained through headspace collection and analysis on GC-MS, dimethylsulfoxide, 1-butanol, and 3-hydroxy-2-butanone (acetoin) were identified. Taken together, these results suggest that B. valezensis G341 can be used as a biocontrol agent for various plant diseases caused by phytopathogenic fungi.
引用
收藏
页码：488 / 498
页数：11
相关论文
共 56 条
[1]   Active package for wild strawberry fruit (Fragaria vesca L.) [J].
Almenar, Eva ;
Del Valle, Valeria ;
Catala, Ramon ;
Gavara, Rafael .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2007, 55 (06) :2240-2245
[2]   Identifying natural volatile compounds that control gray mold (Botrytis cinerea) during postharvest storage of strawberry, blackberry, and grape [J].
Archbold, DD ;
HamiltonKemp, TR ;
Barth, MM ;
Langlois, BE .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1997, 45 (10) :4032-4037
[3]   Effect of volatile compounds produced by Bacillus strains on postharvest decay in citrus [J].
Arrebola, Eva ;
Sivakumar, Dharini ;
Korsten, Lise .
BIOLOGICAL CONTROL, 2010, 53 (01) :122-128
[4]   Biocontrol of Rhizoctonia solani damping-off of tomato with Bacillus subtilis RB14 [J].
Asaka, O ;
Shoda, M .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1996, 62 (11) :4081-4085
[5]   Two novel MvaT-like global regulators control exoproduct formation and biocontrol activity in root-associated Pseudomonas fluorescens CHAO [J].
Baehler, E ;
de Werra, P ;
Wick, LY ;
Péchy-Tarr, M ;
Mathys, S ;
Maurhofer, M ;
Keel, C .
MOLECULAR PLANT-MICROBE INTERACTIONS, 2006, 19 (03) :313-329
[6]  
Barnard M, 1997, INT PEST CONTROL, V39, P161
[7]   IDENTIFICATION OF ANTIBIOTICS OF ITURIN GROUP IN VARIOUS STRAINS OF BACILLUS-SUBTILIS [J].
BESSON, F ;
PEYPOUX, F ;
MICHEL, G ;
DELCAMBE, L .
JOURNAL OF ANTIBIOTICS, 1978, 31 (04) :284-288
[8]   Genomic and metabolic traits endow Bacillus velezensis CC09 with a potential biocontrol agent in control of wheat powdery mildew disease [J].
Cai, Xun-Chao ;
Liu, Chang-Hong ;
Wang, Bao-Tong ;
Xue, Ya-Rong .
MICROBIOLOGICAL RESEARCH, 2017, 196 :89-94
[9]   Isolation and characterization of antagonistic Bacillus subtilis strains from the avocado rhizoplane displaying biocontrol activity [J].
Cazorla, F. M. ;
Romero, D. ;
Pérez-García, A. ;
Lugtenberg, B. J. J. ;
de Vicente, A. ;
Bloemberg, G. .
JOURNAL OF APPLIED MICROBIOLOGY, 2007, 103 (05) :1950-1959
[10]   Biocontrol of avocado dematophora root rot by antagonistic Pseudomonas fluorescens PCL1606 correlates with the production of 2-hexyl 5-propyl resorcinol [J].
Cazorla, FM ;
Duckett, SB ;
Bergström, ET ;
Noreen, S ;
Odijk, R ;
Lugtenberg, BJJ ;
Thomas-Oates, JE ;
Bloemberg, GV .
MOLECULAR PLANT-MICROBE INTERACTIONS, 2006, 19 (04) :418-428
123456