Flow of cerebrospinal fluid is driven by arterial pulsations and is reduced in hypertension

被引：600

作者：

Mestre, Humberto ^{[1
,2
]}

Tithof, Jeffrey ^{[3
]}

Du, Ting ^{[1
,4
]}

Song, Wei ^{[1
]}

Peng, Weiguo ^{[1
,5
]}

Sweeney, Amanda M. ^{[1
]}

Olveda, Genaro ^{[1
]}

Thomas, John H. ^{[3
]}

Nedergaard, Maiken ^{[1
,5
]}

Kelley, Douglas H. ^{[3
]}

机构：

[1] Univ Rochester, Med Ctr, Ctr Translat Neuromed, Rochester, NY 14642 USA

[2] Univ Rochester, Dept Neurosci, Med Ctr, Rochester, NY 14642 USA

[3] Univ Rochester, Dept Mech Engn, Rochester, NY 14627 USA

[4] China Med Univ, Shenyang 110122, Liaoning, Peoples R China

[5] Univ Copenhagen, Fac Hlth & Med Sci, Ctr Translat Neuromed, DK-2200 Copenhagen, Denmark

来源：

NATURE COMMUNICATIONS | 2018年 / 9卷

基金：

欧盟地平线“2020”;

关键词：

BETA-AMYLOID DEPOSITION; INTERSTITIAL FLUID; PERIVASCULAR SPACES; PARTICLE TRACKING; SOLUTE TRANSPORT; BLOOD-PRESSURE; BRAIN; PATHWAYS; RESPIRATION; CIRCULATION;

D O I：

10.1038/s41467-018-07318-3

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Flow of cerebrospinal fluid (CSF) through perivascular spaces (PVSs) in the brain is important for clearance of metabolic waste. Arterial pulsations are thought to drive flow, but this has never been quantitatively shown. We used particle tracking to quantify CSF flow velocities in PVSs of live mice. CSF flow is pulsatile and driven primarily by the cardiac cycle. The speed of the arterial wall matches that of the CSF, suggesting arterial wall motion is the principal driving mechanism, via a process known as perivascular pumping. Increasing blood pressure leaves the artery diameter unchanged but changes the pulsations of the arterial wall, increasing backflow and thereby reducing net flow in the PVS. Perfusion-fixation alters the normal flow direction and causes a 10-fold reduction in PVS size. We conclude that particle tracking velocimetry enables the study of CSF flow in unprecedented detail and that studying the PVS in vivo avoids fixation artifacts.

引用

页数：9