Super-Resolution Imaging Approaches for Quantifying F-Actin in Immune Cells

被引：5

作者：

Garlick, Evelyn ^{[1
,2
,3
]}

Thomas, Steven G. ^{[1
,2
,3
]}

Owen, Dylan M. ^{[2
,3
,4
,5
]}

机构：

[1] Univ Birmingham, Inst Cardiovasc Sci, Coll Med & Dent Sci, Birmingham, W Midlands, England

[2] Univ Birmingham, Ctr Membrane Proteins & Receptors, Midlands, England

[3] Univ Nottingham, Midlands, England

[4] Univ Birmingham, Inst Immunol & Immunotherapy, Coll Med & Dent Sci, Birmingham, W Midlands, England

[5] Univ Birmingham, Sch Math, Coll Engn & Phys Sci, Birmingham, W Midlands, England

来源：

FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY | 2021年 / 9卷

基金：

英国生物技术与生命科学研究理事会;

关键词：

actin; microscopy; super-resolution; fiber analysis; cytoskeleton; IMMUNOLOGICAL SYNAPSE; LOCALIZATION MICROSCOPY; GRANULE SECRETION; DYNAMICS; CYTOSKELETON; ACTIVATION; VISUALIZATION; LIFEACT; PROBES; BINDING;

D O I：

10.3389/fcell.2021.676066

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Immune cells comprise a diverse set of cells that undergo a complex array of biological processes that must be tightly regulated. A key component of cellular machinery that achieves this is the cytoskeleton. Therefore, imaging and quantitatively describing the architecture and dynamics of the cytoskeleton is an important research goal. Optical microscopy is well suited to this task. Here, we review the latest in the state-of-the-art methodology for labeling the cytoskeleton, fluorescence microscopy hardware suitable for such imaging and quantitative statistical analysis software applicable to describing cytoskeletal structures. We also highlight ongoing challenges and areas for future development.</p>

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页数：10

相关论文

共 83 条

[21] The immunological synapse and the actin cytoskeleton: molecular hardware for T cell signaling [J].

Dustin, ML ;

Cooper, JA .

NATURE IMMUNOLOGY, 2000, 1 (01) :23-29

[22] Lifeact-GFP alters F-actin organization, cellular morphology and biophysical behaviour [J].

Flores, Luis R. ;

Keeling, Michael C. ;

Zhang, Xiaoli ;

Sliogeryte, Kristina ;

Gavara, Nuria .

SCIENTIFIC REPORTS, 2019, 9 (1)

[23] A novel universal algorithm for filament network tracing and cytoskeleton analysis [J].

Flormann, Daniel A. D. ;

Schu, Moritz ;

Terriac, Emmanuel ;

Thalla, Divyendu ;

Kainka, Lucina ;

Koch, Marcus ;

Gad, Annica K. B. ;

Lautenschlaeger, Franziska .

FASEB JOURNAL, 2021, 35 (05)

[24] Functional Anatomy of T Cell Activation and Synapse Formation [J].

Fooksman, David R. ;

Vardhana, Santosh ;

Vasiliver-Shamis, Gaia ;

Liese, Jan ;

Blair, David A. ;

Waite, Janelle ;

Sacristan, Catarina ;

Victora, Gabriel D. ;

Zanin-Zhorov, Alexandra ;

Dustin, Michael L. .

ANNUAL REVIEW OF IMMUNOLOGY, VOL 28, 2010, 28 :79-105

[25] Actin-based protrusions of migrating neutrophils are intrinsically lamellar and facilitate direction changes [J].

Fritz-Laylin, Lillian K. ;

Riel-Mehan, Megan ;

Chen, Bi-Chang ;

Lord, Samuel J. ;

Goddard, Thomas D. ;

Ferrin, Thomas E. ;

Nicholson-Dykstra, Susan M. ;

Higgs, Henry ;

Johnson, Graham T. ;

Betzig, Eric ;

Mullins, R. Dyche .

[26] Cytoskeletal actin dynamics shape a ramifying actin network underpinning immunological synapse formation [J].

Fritzsche, Marco ;

Fernandes, Ricardo A. ;

Chang, Veronica T. ;

Colin-York, Huw ;

Clausen, Mathias P. ;

Felce, James H. ;

Galiani, Silvia ;

Erlenkamper, Christoph ;

Santos, Ana M. ;

Heddleston, John M. ;

Pedroza-Pacheco, Isabela ;

Waithe, Dominic ;

de la Serna, Jorge Bernardino ;

Lagerholm, B. Christoffer ;

Liu, Tsung-Li ;

Chew, Teng-Leong ;

Betzig, Eric ;

Davis, Simon J. ;

Eggeling, Christian .

SCIENCE ADVANCES, 2017, 3 (06)

[27] Confined diffusion of transmembrane proteins and lipids induced by the same actin meshwork lining the plasma membrane [J].

Fujiwara, Takahiro K. ;

Iwasawa, Kokoro ;

Kalay, Ziya ;

Tsunoyama, Taka A. ;

Watanabe, Yusuke ;

Umemura, Yasuhiro M. ;

Murakoshi, Hideji ;

Suzuki, Kenichi G. N. ;

Nemoto, Yuri L. ;

Morone, Nobuhiro ;

Kusumi, Akihiro .

MOLECULAR BIOLOGY OF THE CELL, 2016, 27 (07) :1101-1119

[28] Dynamic Bayesian Cluster Analysis of Live-Cell Single Molecule Localization Microscopy Datasets [J].

Griffie, Juliette ;

Burn, Garth L. ;

Williamson, David J. ;

Peters, Ruby ;

Rubin-Delanchy, Patrick ;

Owen, Dylan M. .

SMALL METHODS, 2018, 2 (09)

[29] Fast live-cell conventional fluorophore nanoscopy with ImageJ through super-resolution radial fluctuations [J].

Gustafsson, Nils ;

Culley, Sian ;

Ashdown, George ;

Owen, Dylan M. ;

Pereira, Pedro Matos ;

Henriques, Ricardo .

NATURE COMMUNICATIONS, 2016, 7

[30] Cluster size regulates protein sorting in the immunological synapse [J].

Hartman, Nina C. ;

Nye, Jeffrey A. ;

Groves, Jay T. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2009, 106 (31) :12729-12734

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