Effects of alkaline solvents and heating temperatures on the solubilization and degradation of gel-forming Extracellular Polymeric Substances (EPS) extracted from aerobic granular sludge

被引：11

作者：

Bou-Sarkis, Abdo ^{[1
,2
]}

Pagliaccia, Benedetta ^{[2
,3
]}

Ric, Audrey ^{[4
]}

Derlon, Nicolas ^{[5
]}

Paul, Etienne ^{[2
]}

Bessiere, Yolaine ^{[2
]}

Girbal-Neuhauser, Elisabeth ^{[1
]}

机构：

[1] Univ Toulouse, Inst Univ Technol, Lab Biotechnol Agroalimentaire & Environm, LBAE,UPS,URU 456, F-32000 Auch, France

[2] Univ Toulouse, INP TBI, UPS, INSA, F-31077 Toulouse, France

[3] Univ Florence, Dept Civil & Environm Engn, Via Santa Marta 3, I-50139 Florence, FI, Italy

[4] Univ Toulouse, Toulouse Field Flow Fractionat Ctr TFFFC, INP Ecole Ingn PURPAN, F-31076 Toulouse 3, France

[5] Swiss Fed Inst Aquat Sci & Technol, Dept Proc Engn, EAWAG, CH-8600 Dubendorf, Switzerland

来源：

BIOCHEMICAL ENGINEERING JOURNAL | 2022年 / 185卷

关键词：

Extracellular polymeric substances (EPS); Aerobic granular sludge; Alkaline solubilization; Heating temperature; Precipitation; Gel-forming polymers; QUANTIFICATION; RECOVERY; PROTOCOL;

D O I：

10.1016/j.bej.2022.108500

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The effects of solvents and heating temperatures on the recovery and degradation of gel-forming EPS (Extracellular Polymeric Substances) extracted from aerobic granular sludge were investigated. Sodium hydroxide has allowed a better EPS solubilization compared to sodium carbonate (711.3 +/- 38 and 408.4 +/- 4 mg VS/g VS respectively) but the capacity to precipitate gel-forming EPS at acidic pH was higher in sodium carbonate (22% compared to 6% in sodium hydroxide at 80 degrees C). Increasing the temperature from 20 degrees C to 80 degrees C during EPS solubilization has multiplied the amount of solubilized matter by 4 in sodium carbonate and 2.2 in sodium hydroxide. Similar biochemical composition but different molecular weight profiles were observed for the two solubilized EPS extracts, evidencing partial degradation of the polymers in sodium hydroxide. Furthermore, coupling molecular weight analysis and precipitation yield measurement revealed that the lower precipitation of sodium hydroxide solubilized EPS is related to the size reduction due to degradation. Altogether these results help to guide towards a unified protocol for the extraction of gel-forming EPS.

引用

页数：9

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