DNA can interfere with detection of Borrelia burgdorferi in skin biopsy specimens by PCR

被引：66

作者：

Cogswell, FB ^{[1
]}

Bantar, CE ^{[1
]}

Hughes, TG ^{[1
]}

Gu, Y ^{[1
]}

Philipp, MT ^{[1
]}

机构：

[1] TULANE UNIV,TULANE PRIMATE RES CTR,MED CTR,DEPT PARASITOL,COVINGTON,LA 70433

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1996年 / 34卷 / 04期

关键词：

D O I：

10.1128/JCM.34.4.980-982.1996

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

It is demonstrated that a diagnostic PCR for Borrelia burgdorferi can be inhibited in the presence of more than 500 ng of host (monkey skin) DNA. The Inhibitor is the host DNA itself. An acceptable value for analytical sensitivitg can be obtained by diluting the skin-B. burgdorferi proteinase K lysate to a level below the inhibitory concentration of the host DNA. Dilution of the lysate may obviate the need for further DNA purification.

引用

页码：980 / 982

页数：3

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