Functional analysis of polygalacturonase gene RsPG2 from Rhizoctonia solani, the pathogen of rice sheath blight

被引：22

作者：

Chen, Xijun ^{[1
,2
]}

Li Lili ^{[1
]}

Zhang, Yun ^{[1
]}

Zhang, Jiahao ^{[1
]}

Ouyang, Shouqiang ^{[1
]}

Zhang, Qingxia ^{[1
]}

Tong, Yunhui ^{[1
]}

Xu, Jingyou ^{[1
]}

Zuo, Shimin ^{[2
]}

机构：

[1] Yangzhou Univ, Hort & Plant Protect Coll, Yangzhou 225009, Jiangsu, Peoples R China

[2] Yangzhou Univ, Key Lab Crop Genet & Physiol, Coinnovat Ctr Modern Prod Technol Grain Crops, Key Lab Plant Funct Genom,Minist Educ, Yangzhou 225009, Jiangsu, Peoples R China

来源：

EUROPEAN JOURNAL OF PLANT PATHOLOGY | 2017年 / 149卷 / 02期

关键词：

Functional characterization; pg gene; R; solani; ACIDIC ENDO-POLYGALACTURONASE; INHIBITING PROTEINS PGIPS; 1ST REPORT; EXPRESSION; RESISTANCE; ENDOPOLYGALACTURONASE; IDENTIFICATION; DEFENSE; CLONING; PLANTS;

D O I：

10.1007/s10658-017-1198-5

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Rice sheath blight, which is caused by Rhizoctonia solani, is a devastating disease in many areas of the world where rice is cultivated. The role of polygalacturonase (PG) in the infection process of R. solani was investigated in the present study by cloning and investigating the function of a new PG-encoding gene, which was designated RsPG2. Bioinformatics showed that RsPG2 consisted of a 1,633 bp open reading frame (ORF) with six introns; the deduced protein contained 436 amino acids with a predicted mass of 45.88 kDa after cleavage of the predicted 17-amino acid signal sequence. Culture supernatants obtained from Pichia pastoris producing recombinant RsPG2 degraded polygalacturonic acid in vitro, thus confirming that RsPG2 encodes a PG gene. Purified recombinant RsPG2 also degraded rice tissue and elicited obvious necrotic symptoms 48 h after inoculation. Real-time qRT-PCR indicated that the expression of RsPG2 was strongly induced during the infection of rice by R. solani. These data unequivocally demonstrate that RsPG2 plays an important role in R. solani infection and provide further insights for elucidating the function of pg genes in the virulence of R. solani.

引用

页码：491 / 502

页数：12

共 47 条

[11] NPS@:: Network Protein Sequence Analysis [J].

Combet, C ;

Blanchet, C ;

Geourjon, C ;

Deléage, G .

TRENDS IN BIOCHEMICAL SCIENCES, 2000, 25 (03) :147-150

[12] On filtering false positive transmembrane protein predictions [J].

Cserzö, M ;

Eisenhaber, F ;

Eisenhaber, B ;

Simon, I .

PROTEIN ENGINEERING, 2002, 15 (09) :745-752

[13] The role of polygalacturonase-inhibiting proteins (PGIPS) in defense against pathogenic fungi [J].

De Lorenzo, G ;

D'Ovidio, R ;

Cervone, F .

ANNUAL REVIEW OF PHYTOPATHOLOGY, 2001, 39 :313-335

[14] Prediction of Protein Solubility in Escherichia coli Using Logistic Regression [J].

Diaz, Armando A. ;

Tomba, Emanuele ;

Lennarson, Reese ;

Richard, Rex ;

Bagajewicz, Miguel J. ;

Harrison, Roger G. .

BIOTECHNOLOGY AND BIOENGINEERING, 2010, 105 (02) :374-383

[15] Isolation and Heterologous Expression of a Polygalacturonase Produced by Fusarium oxysporum f. sp cubense Race 1 and 4 [J].

Dong, Zhangyong ;

Wang, Zhenzhong .

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2015, 16 (04) :7595-7607

[16] Identification and pathogenicity of Rhizoctonia solani and binucleate Rhizoctonia anastomosis groups isolated from forage legumes in Erzurum, Turkey [J].

Eken, C ;

Demirci, E .

PHYTOPARASITICA, 2003, 31 (01) :76-80

[17] Aspergillus kawachii produces an acidic pectin releasing enzyme activity [J].

Esquivel, JCC ;

Hours, RA ;

Voget, CE ;

Mignone, CF .

JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 1999, 88 (01) :48-52

[18] Structural requirements of endopolygalacturonase for the interaction with PGIP (polygalacturonase-inhibiting protein) [J].

Federici, L ;

Caprari, C ;

Mattei, B ;

Savino, C ;

Di Matteo, A ;

De Lorenzo, G ;

Cervone, F ;

Tsernoglou, D .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (23) :13425-13430

[19] Transgenic expression of polygalacturonase-inhibiting proteins in Arabidopsis and wheat increases resistance to the flower pathogen Fusarium graminearum [J].

Ferrari, S. ;

Sella, L. ;

Janni, M. ;

De Lorenzo, G. ;

Favaron, F. ;

D'Ovidio, R. .

PLANT BIOLOGY, 2012, 14 :31-38

[20] Transgenic expression of a fungal endo-polygalacturonase increases plant resistance to pathogens and reduces auxin sensitivity [J].

Ferrari, Simone ;

Galletti, Roberta ;

Pontiggia, Daniela ;

Manfredini, Cinzia ;

Lionetti, Vincenzo ;

Bellincampi, Daniela ;

Cervone, Felice ;

De Lorenzo, Giulia .

PLANT PHYSIOLOGY, 2008, 146 (02) :669-681

← 1 2 3 4 5 →