Multiparametric color-changing fluorescence probes

被引：78
作者：
Demchenko, AP [1 ]
Klymchenko, AS
Pivovarenko, VG
Ercelen, S
Duportail, G
Mely, Y
机构：
[1] AV Palladin Biochem Inst, UA-01030 Kiev, Ukraine
[2] TUBITAK, Int Ctr Genet Engn & Biotechnol, TR-41470 Gebze, Turkey
[3] Kyiv Natl Taras Shevchenko Univ, Fac Chem, UA-01033 Kiev, Ukraine
[4] Univ Strasbourg 1, Fac Pharm, F-67401 Illkirch Graffenstaden, France
来源：
JOURNAL OF FLUORESCENCE | 2003年 / 13卷 / 04期
关键词：
site-sensitive probes; steady-state fluorescence; multiparametric analysis; excited-state intramolecular proton transfer;
D O I：
10.1023/A:1025302525771
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The possibility of obtaining rich multiparametric information about various intermolecular interactions in the probe environment was demonstrated using single fluorescence probe. It was shown that in most cases the probe molecules exist as single excited-state and single ground-state species, and electron transition between them generates only one fluorescence emission band. A systematic analysis of excitation and fluorescence spectra of probe FE obtained in 21 representative solvents enabled to develop a multiparameteric approach for the simultaneous analysis of various physical-chemical properties of probe microenvironments. It was stated that the information can be obtained by simple steady-state spectroscopic measurements.
引用
收藏
页码：291 / 295
页数：5
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