ZEB2 and ZEB1 expression in a spontaneous canine model of invasive micropapillary carcinoma of the mammary gland

被引：12
|
作者：
Gamba, C. O. [1 ]
Campos, L. C. [1 ]
Negreiros-Lima, G. L. [2 ]
Maciel-Lima, K. [2 ]
Sousa, L. P. [2 ]
Estrela-Lima, A. [3 ]
Ferreira, E. [1 ]
Cassali, G. D. [1 ]
机构：
[1] Univ Fed Minas Gerais, Dept Patol Geral, Inst Ciencia Biol, BR-31270901 Belo Horizonte, MG, Brazil
[2] Univ Fed Minas Gerais, Dept Anal Clin & Toxicol, Fac Farm, BR-31270901 Belo Horizonte, MG, Brazil
[3] Univ Fed Bahia, Escola Med Vet, Dept Patol & Clin, BR-40170110 Salvador, BA, Brazil
来源：
RESEARCH IN VETERINARY SCIENCE | 2014年 / 97卷 / 03期
关键词：
Canine; Mammary gland; Invasive micropapillary carcinoma; Immunohistochemistry; ZEB1; ZEB2; EPITHELIAL-MESENCHYMAL TRANSITION; TRANSCRIPTION FACTOR ZEB1; E-CADHERIN REPRESSORS; BREAST; SIP1; CANCER; TWIST; SNAIL; SLUG; DIAGNOSIS;
D O I：
10.1016/j.rvsc.2014.09.016
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
ZEB1 and ZEB2 have been recently related to cancer prognosis. We investigated their expression and its association with clinicopathological parameters and overall survival in invasive micropapillary carcinoma (IMPC), which is a metastasising neoplasm of the canine mammary gland. Immunohistochemical evaluation showed nuclear and cytoplasmic staining for ZEB2 and nuclear staining for ZEB1. 'In situ' areas presented higher positivity for cytoplasmic ZEB2 than invasive areas of IMPC did (p = 0.03). ZEB1 positivity was associated with a low histological grade (p = 0.01). A shorter overall survival rate was observed in IMPCs that were positive for cytoplasmic ZEB2 (p = 0.04). Antibodies specificity in canine species was confirmed by western blot. Our results indicated that cytoplasmic ZEB2 appears to be an important factor in the early stages of malignancy and predicts a poor overall survival rate for IMPC in this canine mammary cancer model. ZEB1 downregulation appears to be associated with the dedifferentiation process of IMPC. (C) 2014 Elsevier Ltd. All rights reserved.
引用
收藏
页码：554 / 559
页数：6
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