Dual dye-labeled G-quadruplex aptasensor for detection of thallium(I) using ratiometric fluorescence resonance energy transfer

被引：12

作者：

Li, Tongtong ^{[1
,2
]}

Zhang, Yan ^{[1
,2
]}

Sun, Xiaohong ^{[1
]}

Zhang, Yanjin ^{[1
]}

Wang, Yongan ^{[1
]}

Nie, Zhiyong ^{[1
]}

机构：

[1] Acad Mil Med Sci, Inst Pharmacol & Toxicol, State Key Lab Toxicol & Med Countermeasures, Beijing 100850, Peoples R China

[2] Tianjin Univ, Sch Chem Engn & Technol, Tianjin 300350, Peoples R China

来源：

TALANTA | 2021年 / 233卷

关键词：

Fluorescence sensor; Aptamer; Thallium detection; Fluorescence resonance energy transfer; G-quadruplex; ULTRASENSITIVE DETECTION; APTAMER PROBE; WATER; DNA; LEAD(II); SEQUENCE;

D O I：

10.1016/j.talanta.2021.122508

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A fluorescent probe was developed for ratiometric detection of thallium ions in mineral water samples by modifying a G-rich aptamer (PS2.M 7) with a fluorescence donor (Cyanine-3, Cy-3) and a quencher (Cyanine-5, Cy-5). The probe had a random coil structure that changed into a G-quadruplex structure upon binding with Tl+. This change in structure decreased the distance between the donor and acceptor moieties, which resulted in fluorescence resonance energy transfer between Cy-3 and Cy-5. Under optimized conditions, the limit of detection and linear concentration range for Tl+ were 30.1 mu M (3 sigma) and 10 mu M-10 mM (R-2 = 0.9981), respectively. This simple and cost-effective fluorescence sensor provided satisfactory results for detection of thallium ions in spiked mineral water samples.

引用

页数：7

共 27 条

[1] Human telomeric sequence forms a hybrid-type intramolecular G-quadruplex structure with mixed parallel/antiparallel strands in potassium solution [J].