Unusual degradation behavior of citric acid-crosslinked gelatin in vitro and in vivo

被引：17
|
作者：
Inoue, Motoki [2 ]
Sasaki, Makoto [2 ,3 ]
Taguchi, Tetsushi [1 ,2 ,3 ]
机构：
[1] Natl Inst Mat Sci, Int Ctr Mat Nanoarchitecton MANA, Tsukuba, Ibaraki 3050044, Japan
[2] Natl Inst Mat Sci, Ctr Biomat, Tsukuba, Ibaraki 3050044, Japan
[3] Univ Tsukuba, Grad Sch Pure & Appl Sci, Tsukuba, Ibaraki 3058577, Japan
来源：
POLYMER DEGRADATION AND STABILITY | 2010年 / 95卷 / 10期
关键词：
Gelatin; Matrices; Citric acid; Biodegradation; Crosslinking density; CORONARY-ARTERY; ELUTING STENTS; GLUTARALDEHYDE; HYDROGELS; COLLAGEN; TISSUE;
D O I：
10.1016/j.polymdegradstab.2010.06.029
中图分类号：
O63 [高分子化学（高聚物）];
学科分类号：
070305 ; 080501 ; 081704 ;
摘要：
The aim of this study was to evaluate the biodegradability and biocompatibility of gelatin matrices designed for drug-eluting stents (DESs). The matrices were prepared by crosslinking alkali-treated gelatin (AlGelatin) with a citric acid-based crosslinker, trisuccinimidyl citrate (TSC), to form AlGelatin-TSC. The biodegradation behavior of the matrices was evaluated in vitro and in vivo. An in vitro enzymatic degradation test showed that AlGelatin-TSC prepared at a TSC concentration of 20 mM is the most stable in collagenase solution compared to AlGelatin-TSC prepared at TSC concentrations higher or lower than 20 mM. Then, AlGelatin-TSC were implanted subcutaneously in rats to evaluate their biodegradability and tissue reaction in vivo. Similar to the in vitro degradation behavior, AlGelatin-TSC with TSC concentration of 20 mM exhibited the lowest biodegradable rate in vivo among all AlGelatin-TSC. In addition, strong inflammation and calcification were not observed for AlGelatin-TSC at any TSC concentration. From an analysis of the crosslinking density of the resulting AlGelatin-TSC, the lowest biodegradability of AlGelatin TSC with TSC concentration of 20 mM was due to the highest crosslinking density of the matrix. These results suggest that AlGelatin-TSC is suitable for use as matrices in DESs because of its excellent biocompatibility and biodegradability. (C) 2010 Elsevier Ltd. All rights reserved.
引用
收藏
页码：2088 / 2092
页数：5
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